Myosin Va enhances secretion of herpes simplex virus 1 virions and cell surface expression of viral glycoproteins.

Journal of Virology
Kari L Roberts, Joel D Baines

Abstract

The final step in the egress of herpes simplex virus (HSV) virions requires virion-laden vesicles to bypass cortical actin and fuse with the plasma membrane, releasing virions into the extracellular space. Little is known about the host or viral proteins involved. In the current study, we noted that the conformation of myosin Va (myoVa), a protein known to be involved in melanosome and secretory granule trafficking to the plasma membrane in melanocytes and neuroendocrine cells, respectively, was altered by 4 h after infection with HSV-1 such that an N-terminal epitope expected to be masked in its inactive state was rendered immunoreactive. Wild-type myoVa localized throughout the cytoplasm and to a limited extent in the nuclei of HSV-infected cells. Two different dominant negative myoVa molecules containing cargo-binding domains but lacking the lever arms and actin-binding domains colocalized with markers of the trans-Golgi network (TGN). Expression of dominant negative myoVa isoforms reduced secretion of HSV-1 infectivity into the medium by 50 to 75%, reduced surface expression of glycoproteins B, M, and D, and increased intracellular virus infectivity to levels consistent with increased retention of virions in the cytoplasm. ...Continue Reading

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Citations

Apr 28, 2011·Nature Reviews. Microbiology·Matthew P TaylorLynn W Enquist
Oct 14, 2011·Viruses·Kari L Roberts, Joel D Baines
Oct 14, 2011·Viruses·Sofia V ZaichickGregory A Smith
Feb 26, 2013·Viruses·Tal Kramer, Lynn W Enquist
Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Aug 1, 2015·Experimental Cell Research·Beata FuchsovaPrimal de Lanerolle
Sep 1, 2011·The EMBO Journal·Mark P Dodding, Michael Way
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Jul 19, 2012·Traffic·Daniel HenaffRoger Lippé
Feb 24, 2018·Viruses·Monica Miranda-SaksenaAnthony L Cunningham
Feb 14, 2018·Viruses·Christopher E DenesRussell J Diefenbach
Jan 5, 2018·Journal of Virology·Adrian R WilkieDonald M Coen
Nov 28, 2014·Journal of Virology·Jun AriiYasushi Kawaguchi
Nov 28, 2014·Journal of Virology·Kari L RobertsRobert A Lamb
Apr 25, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Liran Ben YaakovAbraham Minsky
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Nov 6, 2018·Frontiers in Microbiology·Francisco J IbáñezPablo A González
Sep 23, 2018·Protein & Cell·Ying LvHongyu Deng
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Oct 30, 2019·Microorganisms·Raghuram KogantiDeepak Shukla
Mar 9, 2021·Frontiers in Microbiology·Chunmei LiBin Tian

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