Myosin Va is proteolysed in rat cerebellar granule neurons after excitotoxic injury

Neuroscience Letters
Silvestre AlavezL C Cameron

Abstract

Cerebellar granule neurons when exposed to glutamate die through an excitotoxic mechanism induced by overactivation of glutamate receptors. This kind of cell death is mediated by an overload of intracellular calcium involving calpain activation, a Ca2+ -dependent intracellular cysteine protease, among other intracellular responses. On the other hand, class V myosins are proteins that move cargo along actin filaments and one of its members, myosin Va, is involved in vesicles transport. Here we studied the effect of excitotoxicity on myosin Va in cultured cerebellar granule neurons. Western blot analysis of control cultures shows a band corresponding to myosin Va as well as an 80 kDa band corresponding to its proteolytic product by calpain. When cells are exposed to glutamate (500 microM), kainate (100 microM) or NMDA (150 microM) during 3-24 h, the proteolytic processing of myosin Va is markedly increased. This proteolysis is inhibited by leupeptin (100 microM) and calpain inhibitor I (50 microM). These inhibitors also significantly improve the morphological appearance of the neurons possibly through the preservation of the cytoskeleton integrity. Our results suggest that myosin Va is a target for calpain I during an excitotoxic...Continue Reading

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Citations

Jun 10, 2005·FEBS Letters·Marta Artal-Sanz, Nektarios Tavernarakis
Sep 9, 2006·CNS Drug Reviews·Antoni CaminsMercè Pallàs
Nov 17, 2010·Journal of Neurochemistry·Rüdiger RudolfHans-Hermann Gerdes
Apr 22, 2009·Brain Research·Sheila Cristina de Souza MartinsVivaldo Moura-Neto
Jul 2, 2011·Journal of Insect Physiology·Luciana Karen CalábriaFoued Salmen Espindola
Jan 11, 2014·Biochemical and Biophysical Research Communications·Adriana Bassini, L C Cameron

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