Myosin VI regulates endocytosis of the cystic fibrosis transmembrane conductance regulator.

The Journal of Biological Chemistry
Agnieszka Swiatecka-UrbanBruce A Stanton

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP-regulated Cl(-) channel expressed in the apical plasma membrane in fluid-transporting epithelia. Although CFTR is rapidly endocytosed from the apical membrane of polarized epithelial cells and efficiently recycled back to the plasma membrane, little is known about the molecular mechanisms regulating CFTR endocytosis and endocytic recycling. Myosin VI, an actin-dependent, minus-end directed mechanoenzyme, has been implicated in clathrin-mediated endocytosis in epithelial cells. The goal of this study was to determine whether myosin VI regulates CFTR endocytosis. Endogenous, apical membrane CFTR in polarized human airway epithelial cells (Calu-3) formed a complex with myosin VI, the myosin VI adaptor protein Disabled 2 (Dab2), and clathrin. The tail domain of myosin VI, a dominant-negative recombinant fragment, displaced endogenous myosin VI from interacting with Dab2 and CFTR and increased the expression of CFTR in the plasma membrane by reducing CFTR endocytosis. However, the myosin VI tail fragment had no effect on the recycling of endocytosed CFTR or on fluid-phase endocytosis. CFTR endocytosis was decreased by cytochalasin D, an actin-filament dep...Continue Reading

References

Aug 1, 1992·The American Journal of Physiology·C M Fuller, D J Benos
Apr 23, 1992·Nature·S A KuznetsovD G Weiss
Feb 1, 1989·The Journal of Cell Biology·J E Heuser, R G Anderson
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L S PrinceR B Marchase
Jul 1, 1994·The American Journal of Physiology·E M SchwiebertB A Stanton
Aug 1, 1994·The American Journal of Physiology·P WebsterC R Marino
Oct 1, 1994·The Journal of Cell Biology·T Hasson, M S Mooseker
Jun 16, 1997·The Journal of Cell Biology·T HassonD P Corey
Jun 27, 1998·The Journal of Biological Chemistry·R A WarrenC A Enns
Oct 1, 1998·The American Journal of Physiology·J LoffingB A Stanton
Mar 10, 1999·The American Journal of Physiology·C H MitchellM M Civan
Oct 16, 1999·Nature·A L WellsH L Sweeney
Oct 20, 1999·Developmental Biology·T SelfK P Steel
Nov 24, 1999·The Journal of Clinical Investigation·B D MoyerB A Stanton
Jan 25, 2000·Experimental Eye Research·J BrecklerB Burnside
Jun 30, 2000·Current Opinion in Cell Biology·K E MostovY Altschuler
Jul 8, 2000·Trends in Cell Biology·O C Rodriguez, R E Cheney
Jul 27, 2000·American Journal of Physiology. Cell Physiology·M HowardO A Weisz
Sep 26, 2000·American Journal of Physiology. Cell Physiology·J LoffingB A Stanton
Dec 21, 2000·American Journal of Physiology. Cell Physiology·G D HedaC R Marino
Nov 8, 2001·Experimental Cell Research·L LanzettiG Scita
Dec 1, 2001·FEBS Letters·F BussJ Kendrick-Jones
Jan 10, 2002·Biochemical and Biophysical Research Communications·So NishikawaMitsuo Ikebe
Mar 22, 2002·Biochemical and Biophysical Research Communications·Akira InoueMitsuo Ikebe
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Daniel BiemesderferTama Hasson
Aug 9, 2002·The Journal of Biological Chemistry·Agnieszka Swiatecka-UrbanBruce A Stanton
Nov 28, 2002·Traffic·Folma BussJohn Kendrick-Jones

❮ Previous
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Citations

May 26, 2006·Nature Reviews. Molecular Cell Biology·William B Guggino, Bruce A Stanton
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Thierry Soldati, Manfred Schliwa
Sep 21, 2007·Molecular Biology of the Cell·Susan D ArdenFolma Buss
Nov 1, 2011·Human Molecular Genetics·Julien ColasAleksander Edelman
Dec 18, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Magdalena SobczakMaria Jolanta Redowicz
Feb 29, 2008·Annual Review of Biochemistry·John R Riordan
Feb 16, 2005·Annual Review of Physiology·John R Riordan
Sep 25, 2009·American Journal of Physiology. Cell Physiology·Judith BlaineMoshe Levi
Nov 2, 2012·American Journal of Physiology. Cell Physiology·Alexander NixonNeil A Bradbury
Mar 25, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sophie Moreau-MarquisBruce A Stanton
Mar 11, 2011·PLoS Biology·Tommy L LewisDon B Arnold
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Samia N NaccacheArie Horowitz
Jun 15, 2006·The Journal of Membrane Biology·A J RicciS M Van Netten
Feb 3, 2016·Frontiers in Chemistry·Carlos M FarinhaPeter Jordan
Apr 24, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeshi InoueKazuo Sutoh
Aug 19, 2006·Cell Motility and the Cytoskeleton·Samia N Naccache, Tama Hasson
Feb 11, 2012·Traffic·Sylvain LoubéryEvelyne Coudrier
Oct 18, 2012·British Journal of Pharmacology·Stefania MonterisiManuela Zaccolo
Nov 14, 2006·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Nadia AmeenNeil A Bradbury
Feb 20, 2007·Biochimica Et Biophysica Acta·Tsukasa Okiyoneda, Gergely L Lukacs
Nov 7, 2006·Biochimica Et Biophysica Acta·Radhika GaneshanKevin L Kirk
Apr 2, 2014·The International Journal of Biochemistry & Cell Biology·Aleksander Edelman
May 18, 2013·Biochimica Et Biophysica Acta·David F Steele, David Fedida
Oct 5, 2016·Cellular and Molecular Life Sciences : CMLS·Carlos M Farinha, Sara Canato
May 7, 2013·International Journal of Molecular Sciences·Dmitri V Kravtsov, Nadia A Ameen
Apr 11, 2018·Molecular Brain·János BenczeTibor Hortobágyi
May 24, 2005·Cell Motility and the Cytoskeleton·Magdalena DominikMaria Jolanta Redowicz
Jul 19, 2005·Physiology·Mira Krendel, Mark S Mooseker
Aug 19, 2005·Journal of the American Society of Nephrology : JASN·Li E YangAlicia A McDonough
Aug 24, 2006·American Journal of Physiology. Renal Physiology·C MazzochiP R Smith
Mar 14, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mark W MuschLeon Goldstein

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