Myosin X regulates neuronal radial migration through interacting with N-cadherin

Frontiers in Cellular Neuroscience
Mingming LaiXiaojuan Zhu

Abstract

Proper brain function depends on correct neuronal migration during development, which is known to be regulated by cytoskeletal dynamics and cell-cell adhesion. Myosin X (Myo10), an uncharacteristic member of the myosin family, is an important regulator of cytoskeleton that modulates cell motilities in many different cellular contexts. We previously reported that Myo10 was required for neuronal migration in the developing cerebral cortex, but the underlying mechanism was still largely unknown. Here, we found that knockdown of Myo10 expression disturbed the adherence of migrating neurons to radial glial fibers through abolishing surface Neuronal cadherin (N-cadherin) expression, thereby impaired neuronal migration in the developmental cortex. Next, we found Myo10 interacted with N-cadherin cellular domain through its FERM domain. Furthermore, we found knockdown of Myo10 disrupted N-cadherin subcellular distribution and led to localization of N-cadherin into Golgi apparatus and endosomal sorting vesicle. Taking together, these results reveal a novel mechanism of Myo10 interacting with N-cadherin and regulating its cell-surface expression, which is required for neuronal adhesion and migration.

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Citations

Nov 21, 2015·Journal of Cellular Physiology·Bor Luen Tang
Nov 12, 2016·Current Opinion in Cell Biology·Meredith L WeckMatthew J Tyska
Jan 16, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bo WangWen-Cheng Xiong
Jul 16, 2020·The Journal of Cell Biology·Ulrike TheisenReinhard W Köster
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Isabel Martinez-Garay
Mar 2, 2021·The EMBO Journal·Diégo Cordero Cervantes, Chiara Zurzolo
Apr 19, 2021·Current Opinion in Cell Biology·Chiara Zurzolo

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
PCR
electrophoresis
immunoprecipitation assay
pull-down
co-immunoprecipitats
pulled
Co-immunoprecipitation
FACS

Software Mentioned

FV10
ASW
ImageJ

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