Myosin-XVa Controls Both Staircase Architecture and Diameter Gradation of Stereocilia Rows in the Auditory Hair Cell Bundles.

Journal of the Association for Research in Otolaryngology : JARO
Shadan HadiGregory I Frolenkov

Abstract

Mammalian hair cells develop their mechanosensory bundles through consecutive phases of stereocilia elongation, thickening, and retraction of supernumerary stereocilia. Many molecules involved in stereocilia elongation have been identified, including myosin-XVa. Significantly less is known about molecular mechanisms of stereocilia thickening and retraction. Here, we used scanning electron microscopy (SEM) to quantify postnatal changes in number and diameters of the auditory hair cell stereocilia in shaker-2 mice (Myo15sh2) that lack both "long" and "short" isoforms of myosin-XVa, and in mice lacking only the "long" myosin-XVa isoform (Myo15∆N). Previously, we observed large mechanotransduction current in young postnatal inner (IHC) and outer (OHC) hair cells of both these strains. Stereocilia counts showed nearly identical developmental retraction of supernumerary stereocilia in control heterozygous, Myo15sh2/sh2, and Myo15∆N/∆N mice, suggesting that this retraction is largely unaffected by myosin-XVa deficiency. However, myosin-XVa deficiency does affect stereocilia diameters. In control, the first (tallest) and second row stereocilia grow in diameter simultaneously. However, the third row stereocilia in IHCs grow only until p...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·L G TilneyD J DeRosier
Jan 1, 1986·Hearing Research·L G Tilney, M S Tilney
Jul 1, 1980·The Journal of Cell Biology·L G TilneyM J Mulroy
Dec 8, 1994·The Journal of Comparative Neurology·J A KaltenbachT H Simpson
Aug 23, 2002·Nature·Mark E SchneiderBechara Kachar
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Julia GorelikYuri E Korchev
Sep 11, 2003·Human Molecular Genetics·I Jill KarolyiSally A Camper
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inna A BelyantsevaThomas B Friedman
Mar 17, 2004·The Journal of Cell Biology·Agnieszka K RzadzinskaBechara Kachar
Mar 19, 2005·The Journal of Comparative Neurology·Richard J GoodyearGuy P Richardson
Sep 16, 2006·The Journal of Physiology·Ruben StepanyanGregory I Frolenkov
Oct 6, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark E SchneiderBechara Kachar
Feb 28, 2007·Cell Motility and the Cytoskeleton·Mette M MogensenKaren P Steel
Mar 31, 2009·Nature Neuroscience·Maryline BeurgAnthony J Ricci
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ruben Stepanyan, Gregory I Frolenkov
May 28, 2009·Nature Neuroscience·Christine Petit, Guy P Richardson
Dec 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anthony W PengStefan Heller
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Elisa CaberlottoChristine Petit
Jan 24, 2012·Current Biology : CB·Raymond C MerrittBechara Kachar
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·David N FurnessWalter Marcotti
Apr 22, 2015·Nature Communications·Meghan C DrummondThomas B Friedman
Aug 1, 2015·Molecular Biology of the Cell·Peter-G Barr-Gillespie
Jan 13, 2016·The Journal of Cell Biology·Andrea LelliChristine Petit
Nov 5, 2016·The Journal of Cell Biology·Jocelyn F KreyPeter G Barr-Gillespie
Jul 1, 2017·Nature Communications·Arnaud P J GieseZubair M Ahmed
Mar 5, 2019·Current Biology : CB·Abigail L D TadenevBasile Tarchini
Jan 7, 2020·Current Biology : CB·Jocelyn F KreyPeter G Barr-Gillespie

❮ Previous
Next ❯

Citations

Jan 6, 2021·Current Biology : CB·Jamis McGrathBenjamin J Perrin
May 26, 2021·Current Biology : CB·Anne Houdusse, Margaret A Titus
Nov 9, 2021·Frontiers in Cell and Developmental Biology·Maryna V IvanchenkoDavid P Corey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.