Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization.

Molecular and Cellular Biology
Thomas LeungL Lim

Abstract

The Rho GTPases play distinctive roles in cytoskeletal reorganization associated with growth and differentiation. The Cdc42/Rac-binding p21-activated kinase (PAK) and Rho-binding kinase (ROK) act as morphological effectors for these GTPases. We have isolated two related novel brain kinases whose p21-binding domains resemble that of PAK whereas the kinase domains resemble that of myotonic dystrophy kinase-related ROK. These approximately 190-kDa myotonic dystrophy kinase-related Cdc42-binding kinases (MRCKs) preferentially phosphorylate nonmuscle myosin light chain at serine 19, which is known to be crucial for activating actin-myosin contractility. The p21-binding domain binds GTP-Cdc42 but not GDP-Cdc42. The multidomain structure includes a cysteine-rich motif resembling those of protein kinase C and n-chimaerin and a putative pleckstrin homology domain. MRCK alpha and Cdc42V12 colocalize, particularly at the cell periphery in transfected HeLa cells. Microinjection of plasmid encoding MRCK alpha resulted in actin and myosin reorganization. Expression of kinase-dead MRCK alpha blocked Cdc42V12-dependent formation of focal complexes and peripheral microspikes. This was not due to possible sequestration of the p21, as a kinase-de...Continue Reading

References

Oct 11, 1990·Nucleic Acids Research·J W GrantS L Church
Dec 8, 1995·The Journal of Biological Chemistry·P D BurbeloA Hall
Nov 24, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Oct 13, 1995·The Journal of Biological Chemistry·S ZhangG M Bokoch
Dec 1, 1994·Trends in Genetics : TIG·N Lamarche, A Hall
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Apr 1, 1996·Current Opinion in Cell Biology·R A Cerione, Y Zheng
Apr 1, 1996·Current Opinion in Cell Biology·C Wittenberg, S I Reed
Sep 20, 1996·The Journal of Biological Chemistry·S KurodaK Kaibuchi
May 1, 1996·Current Biology : CB·J L BrownJ Chant
Jan 1, 1997·Current Biology : CB·D N DrechselM Glotzer
Feb 1, 1997·Current Opinion in Cell Biology·N Tapon, A Hall
Mar 17, 1997·The EMBO Journal·R H ChenD Bar-Sagi
Jun 1, 1997·Molecular and Cellular Biology·R G QiuM Symons

❮ Previous
Next ❯

Citations

Apr 20, 1999·The Journal of Cell Biology·Y FukataK Kaibuchi
Aug 6, 2014·The Journal of Cell Biology·Paolo Armando GagliardiLuca Primo
Oct 8, 2014·Cell Communication and Signaling : CCS·Mathieu UnbekandtMichael F Olson
Jan 27, 2006·The American Journal of Pathology·Benjamin Chaigne-DelalandeElisabeth Génot
Jan 5, 2011·Molecular Ecology·Brent L Lockwood, George N Somero
Nov 18, 2000·Experimental Cell Research·A A SchmitzL Van Aelst
Jan 18, 2006·Biochemical and Biophysical Research Communications·Radek CmejlaJana Cmejlova
Oct 30, 2001·International Journal of Medical Microbiology : IJMM·T Adam
Mar 12, 2015·Schizophrenia Research·Glenn T KonopaskeFrancine M Benes
Nov 4, 1998·Current Biology : CB·C L NeudauerI G Macara
Jul 29, 2006·Diabetes·Katrin SchmelzleForest M White
Jun 2, 2016·Small GTPases·Aaron J Farrugia, Fernando Calvo
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Aug 2, 2016·The International Journal of Biochemistry & Cell Biology·Sunxing YanWeiqiang Li
Jan 13, 2015·Biochemical and Biophysical Research Communications·Ayako KitaReiko Sugiura
Jul 12, 2011·Annual Review of Cell and Developmental Biology·Thomas LecuitEdwin Munro
Feb 4, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Virpi TalmanRaimo K Tuominen
Jul 3, 2009·Cerebral Cortex·Jiabin ChenCharles D Gilbert
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·P D BurbeloS Spiegel
Jan 14, 2017·Molecular Oncology·Pahini PandyaVictoria Sanz-Moreno
May 18, 2000·Microscopy Research and Technique·P MadauleS Narumiya
Dec 8, 2006·Molecular Biology of the Cell·Toshiyuki WatanabeShigenobu Yonemura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.