Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects

PloS One
Siril S BakkeVigdis Aas

Abstract

About 80% of patients with type 2 diabetes are classified as overweight. However, only about 1/3 of severely obese subjects have type 2 diabetes. This indicates that several severely obese individuals may possess certain characteristics that protect them against type 2 diabetes. We therefore hypothesized that this apparent paradox could be related to fundamental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexibility were examined in human myotubes derived from severely obese subjects without (BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity was observed in myotubes from severely obese subjects with type 2 diabetes. Lipolysis rate was higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptability were lower in myotubes from severely obese subjects with type 2 diabetes compared to severely obese non-diabetic subjects. There were no differences in lipid distribution and mRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, or the lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also similar in cells from the two groups. In conclusion, myotubes established from severely obese...Continue Reading

References

Sep 1, 1994·The Journal of Clinical Endocrinology and Metabolism·M OttossonP Björntorp
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·J Y KimJ A Houmard
Mar 30, 2001·Biochimica Et Biophysica Acta·F BeissonV Arondel
Mar 20, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M GasterH D Schrøder
May 29, 2004·American Journal of Physiology. Endocrinology and Metabolism·Luc J C van LoonHans A Keizer
Dec 24, 2004·Diabetes·Vladimir B RitovDavid E Kelley
Jul 12, 2005·The Journal of Clinical Investigation·Barbara UkropcovaSteven R Smith
Jan 7, 2006·Nature Medicine·Simon Smyth, Andrew Heron
Jan 11, 2007·Journal of Lipid Research·A J WensaasS Hallén
Feb 22, 2007·American Journal of Physiology. Endocrinology and Metabolism·Graham P HollowayLawrence L Spriet
Nov 16, 2007·American Journal of Physiology. Endocrinology and Metabolism·Cédric MoroSteven R Smith
Dec 6, 2008·The American Journal of Clinical Nutrition·Ian R Lanza, K Sreekumaran Nair
May 13, 2009·The Journal of Biological Chemistry·Véronic BezaireDominique Langin
Dec 2, 2009·Obesity Surgery·Dag HofsøJøran Hjelmesaeth
Apr 7, 2010·Journal of Lipid Research·N P HessvikG H Thoresen
Apr 16, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Benjamin T BikmanG Lynis Dohm
May 6, 2010·American Journal of Physiology. Endocrinology and Metabolism·Eva CorpeleijnArild C Rustan
Aug 28, 2010·Gerontology·Esther PhielixMichael Roden
Sep 17, 2010·The Journal of Clinical Endocrinology and Metabolism·Johan W E JockenEllen E Blaak
Dec 29, 2010·Biochemical and Biophysical Research Communications·Michael Gaster
Oct 26, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·K E BoyleJ A Houmard
Jun 23, 2012·Trends in Endocrinology and Metabolism : TEM·Paul M Coen, Bret H Goodpaster
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Silvie TimmersPatrick Schrauwen
Oct 6, 2012·The International Journal of Biochemistry & Cell Biology·Flemming Dela, Jørn Wulff Helge
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Jose E GalganiCedric Moro
Aug 31, 2013·Trends in Endocrinology and Metabolism : TEM·Pierre-Marie BadinCedric Moro

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Citations

Jul 6, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Nils Gunnar LøvslettenNataša Nikolić
Jul 30, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Anders E GundersenKai Zou
Jul 6, 2017·Journal of Diabetes Research·Pablo Esteban MoralesAlejandra Espinosa
Sep 24, 2020·American Journal of Physiology. Cell Physiology·Kai ZouJoseph A Houmard
Sep 1, 2021·Obesity·Abel M MengesteJenny Lund

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

SPSS
- Pro Analyzer
Gel
GraphPad
GraphPad Prism

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