Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

PloS One
Alexandra M S SoaresLivio M Costa-Junior

Abstract

Seed exudates are plant-derived natural bioactive compounds consisting of a complex mixture of organic and inorganic molecules. Plant seed exudates have been poorly studied against parasite nematodes. This study was undertaken to identify proteins in the Myracrodruon urundeuva seed exudates and to assess the anthelmintic activity against Haemonchus contortus, an important parasite of small ruminants. M. urundeuva seed exudates (SEX) was obtained after immersion of seeds in sodium acetate buffer. SEX was fractionated with ammonium sulfate at 0-90% concentration to generate the ressuspended pellet (SEXF1) and the supernatant (SEXF2). SEX, SEXF1, and SEXF2 were exhaustively dialyzed against distilled water (cut-off: 12 kDa) and the protein contents determined. Mass spectrometry analyses of SEX, SEXF1, and SEXF2 were done to identify proteins and secondary metabolites. The seed exudates contained protease, protease inhibitor, peptidase, chitinase, and lipases as well as the low molecular weight secondary compounds ellagic acid and quercetin rhamnoside. SEX inhibited H. contortus larval development (LDA) (IC50 = 0.29 mg mL-1), but did not affect larval exsheathment (LEIA). On the other hand, although SEXF1 and SEXF2 inhibited H. con...Continue Reading

References

Nov 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L S MansfieldR H Fetterer
Feb 3, 2004·Journal of Chromatography. a·Lei JiangMichael Fountoulakis
Nov 22, 2005·Journal of Ethnopharmacology·Julio Marcelino MonteiroElba Lúcia Cavalcanti de Amorim
Apr 19, 2011·Annual Review of Phytopathology·Brad DayChristopher J Staiger
Jan 10, 2012·Physiologia Plantarum·Manuel MartínezIsabel Díaz
Mar 17, 2012·Physiologia Plantarum·Edouard Pesquet
Sep 10, 2014·International Journal for Parasitology. Parasites and Wildlife·Hannah RoseEric R Morgan
Oct 1, 2014·Research in Veterinary Science·Fabio Ribeiro BragaJackson Victor Araújo
Jun 3, 2015·Journal of Agricultural and Food Chemistry·Raquel O RochaIlka M Vasconcelos
Jul 23, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Chaweewan KlongsiriwetHervé Hoste
Aug 19, 2015·Parasites & Vectors·Christian HeimSaša Štefanić
Sep 4, 2015·Journal of Applied Microbiology·S SchiltzE Gontier
May 31, 2016·Advances in Parasitology·A Harder
Aug 24, 2017·BMC Complementary and Alternative Medicine·Ítalo Diego Rebouças de AraújoVânia Sousa Andrade
Feb 13, 2018·Food Research International·Irlla Correia Lima LicáSonia Malik

❮ Previous
Next ❯

Citations

Jan 27, 2021·The Journal of Parasitology·Helen Silva RibeiroLívio Martins Costa-Junior
Nov 16, 2019·International Journal of Biological Macromolecules·Antônio J S SousaIlka M Vasconcelos
Mar 13, 2021·Veterinary Parasitology·Irlla Correia Lima LicáLivio Martins Costa-Júnior

❮ Previous
Next ❯

Software Mentioned

GraphPad
LC Solution
GraphPad Prism

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Related Papers

Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
Alexandra Martins Dos Santos SoaresLivio M Costa Junior
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
Ledia Feitosa WanderleyLivio M Costa Junior
Veterinary Parasitology
Andreia F OliveiraWagner Vilegas
© 2022 Meta ULC. All rights reserved