Myricanol rescues dexamethasone-induced muscle dysfunction via a sirtuin 1-dependent mechanism

Journal of Cachexia, Sarcopenia and Muscle
Shengnan ShenLigen Lin

Abstract

Muscle atrophy and weakness are adverse effects of high dose or the sustained usage of glucocorticoids. Loss of mitochondria and degradation of protein are highly correlated with muscle dysfunction. The deacetylase sirtuin 1 (SIRT1) plays a vital role in muscle remodelling. The current study was designed to identify myricanol as a SIRT1 activator, which could protect skeletal muscle against dexamethasone-induced wasting. The dexamethasone-induced atrophy in C2C12 myotubes was evaluated by expression of myosin heavy chain, muscle atrophy F-box (atrogin-1), and muscle ring finger 1 (MuRF1), using western blots. The mitochondrial content and oxygen consumption were assessed by MitoTracker staining and extracellular flux analysis, respectively. Muscle dysfunction was established in male C57BL/6 mice (8-10 weeks old, n = 6) treated with a relatively high dose of dexamethasone (25 mg/kg body weight, i.p., 10 days). Body weight, grip strength, forced swimming capacity, muscle weight, and muscle histology were assessed. The expression of proteolysis-related, autophagy-related, apoptosis-related, and mitochondria-related proteins was analysed by western blots or immunoprecipitation. Myricanol (10 μM) was found to rescue dexamethasone-in...Continue Reading

References

Jul 1, 1994·American Journal of Respiratory and Critical Care Medicine·M DecramerP Rogiers
May 22, 2004·Journal of the American Society of Nephrology : JASN·Seoung Woo LeeWilliam E Mitch
Oct 21, 2005·The New England Journal of Medicine·Turk Rhen, John A Cidlowski
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marco SandriBruce M Spiegelman
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sibylle JägerBruce M Spiegelman
Jul 1, 2008·The Journal of Experimental Biology·Carolyn M EngRichard L Lieber
Feb 13, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J Mark DavisBen Davis
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Oct 30, 2009·American Journal of Physiology. Cell Physiology·Sarah M SenfAndrew R Judge
Dec 1, 2009·Cell Metabolism·Eva MasieroMarco Sandri
Feb 6, 2010·FEBS Letters·Marco Sandri
Feb 19, 2010·The American Journal of Clinical Nutrition·Norleena P GullettThomas R Ziegler
Apr 13, 2011·Aging·Patricia S Pardo, Aladin M Boriek
Sep 6, 2011·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Brendon J Gurd
Oct 4, 2011·Journal of Cachexia, Sarcopenia and Muscle·Thomas Thum, Jochen Springer
Jun 8, 2012·Journal of Cachexia, Sarcopenia and Muscle·Alessandro FanzaniWim Martinet
Jun 12, 2012·The American Journal of Pathology·Marion PaulyBasil J Petrof
Aug 21, 2012·Journal of Molecular and Cellular Cardiology·Vanina Romanello, Marco Sandri
Mar 16, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Yichen Wang, Jeffrey E Pessin
Jul 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Melanie GertzClemens Steegborn
Jun 19, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G H DaiF Yang
Feb 19, 2015·Frontiers in Physiology·Theodore P Braun, Daniel L Marks
Mar 3, 2015·Bone Marrow Transplantation·I AldossV Pullarkat
Jul 3, 2015·Nature Communications·Han DaiJames L Ellis
Sep 25, 2015·Journal of Cachexia, Sarcopenia and Muscle·T Scott BowenVolker Adams
Sep 25, 2015·Journal of Cachexia, Sarcopenia and Muscle·Evelien GielenSabine Verschueren
Oct 31, 2015·Nutrients·Fangbo XiaRuile Pan
Dec 18, 2015·Journal of Cachexia, Sarcopenia and Muscle·Cathleen DrescherJochen Springer
Mar 1, 2016·Mechanisms of Ageing and Development·Yujia YuanYanrong Lu
May 3, 2016·The Journal of Nutritional Biochemistry·Rie MukaiJunji Terao
May 31, 2016·Journal of Cachexia, Sarcopenia and Muscle·Vincenzo MusolinoJochen Springer

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Citations

May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lorenzo Di Cesare MannelliCarla Ghelardini
Aug 23, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shigeto TomiyaKoichi Nakazato
Oct 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yunshao XuLigen Lin
Oct 21, 2020·Biomedicines·Laura C GunderDave Bridges
Dec 5, 2020·Oxidative Medicine and Cellular Longevity·Xueying LiuChuangpeng Shen
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gonçalo P RosaMaria Carmo Barreto
Apr 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Liao ZhiyinXiao Qian
Jul 19, 2021·Biochemical and Biophysical Research Communications·Hengting ChenXinlong Ma
Jan 23, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ziqiu HanZhangbin Gong

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Methods Mentioned

BETA
nuclear translocation
protein assay
immunoprecipitation
acetylation
nuclear

Software Mentioned

Graphpad Prism
PMV
Image Lab
AutoDockTools
GraphPad
autogrid4
CellSens
Python Molecular Viewer ( PMV

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