Myricetin induces apoptosis via endoplasmic reticulum stress and DNA double-strand breaks in human ovarian cancer cells

Molecular Medicine Reports
Ye XuZhixin Li

Abstract

The mechanisms underlying myricetin-induced cancer cell apoptosis remain to be elucidated. Certain previous studies have shown that myricetin induces apoptosis through the mitochondrial pathway. Apoptosis, however, can also be induced by other classical pathways, including endoplasmic reticulum (ER) stress and DNA double‑strand breaks (DSBs). The aim of the present study was to assess whether these two apoptotic pathways are involved in myricetin‑induced cell death in SKOV3 ovarian cancer cells. The results revealed that treatment with myricetin inhibited viability of SKOV3 cells in a dose‑dependent manner. Myricetin induced nuclear chromatin condensation and fragmentation, and also upregulated the protein levels of active caspase 3 in a time‑dependent manner. In addition, myricetin upregulated ER stress‑associated proteins, glucose‑regulated protein‑78 and C/EBP homologous protein in SKOV3 cells. Phosphorylation of H2AX, a marker of DNA DSBs, was revealed to be upregulated in myricetin-treated cells. The data indicated that myricetin induces DNA DSBs and ER stress, which leads to apoptosis in SKOV3 cells.

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Citations

Jan 10, 2019·Basic & Clinical Pharmacology & Toxicology·Stefani StollMárcia Inês Goettert
Dec 1, 2018·Journal of Cellular Physiology·Raedeh SaraeiAli Hassanzadeh
Jul 3, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zhiqing ZhouYanli He
Oct 28, 2019·Mini Reviews in Medicinal Chemistry·Yutao XieYajun Cao
Nov 27, 2019·Biomolecules·Shonia SubramaniamAmmu Kutty Radhakrishnan
Jan 2, 2019·Cancers·Mariam AbotalebDietrich Büsselberg
Aug 3, 2020·BMC Complementary Medicine and Therapies·Yasaman TaheriJavad Sharifi-Rad
Aug 27, 2019·DNA Repair·Maryam MajidiniaBahman Yousefi
Dec 19, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaominting SongJin Pei
Oct 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdelsamed I ElshamyAbdel-Razik H Farrag

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Methods Mentioned

BETA
Protein Assay
electrophoresis
confocal microscopy

Software Mentioned

Quantity One

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