Myristylation of the large surface protein is required for hepatitis B virus in vitro infectivity

Virology
Volker BrussP R Galle

Abstract

The large surface protein (L) of the enveloped hepatitis B virus (HBV) is myristylated at glycine 2. To investigate whether this fatty acid moiety is required for HBV infectivity we made use of a point mutant in which the myristyl acceptor was mutated to a nonfunctional alanine. The mutant virus and a wild-type control were expressed in a human hepatoma cell line by transfection of genomic DNA constructs. Comparable amounts of virions were secreted by both strains as measured by the endogenous polymerase activity of immunoprecipitated virions. The presentation of an N-terminal epitope of L on the virion surface was not influenced by the mutation. To test the infectivity of this mutant virus primary human hepatocytes were incubated with the media of transfected cells. The covalently circular closed HBV DNA molecules generated after infection were discriminated from the open circular DNA genomes of inoculated virions by a sensitive PCR-based technique. The experiments demonstrated that the wild type was infectious but not the myristate negative mutant. This reflects the phenotype of an homologous duck hepatitis B virus mutant although the N-terminal L protein domains of this virus and of HBV show no primary sequence homology.

Citations

May 12, 2007·Journal of Virology·Charlotte LepèrePhilippe Gripon
Sep 11, 2009·Journal of Virology·Charlotte Lepère-DouardPhilippe Gripon
Jul 15, 2005·Journal of Virology·Azeneth BarreraRobert E Lanford
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
Apr 29, 2014·Gastroenterology·Stephan UrbanFabien Zoulim
Nov 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jordan Feld, Stephen Locarnini
Mar 31, 2004·Trends in Microbiology·Sebastian Maurer-Stroh, Frank Eisenhaber
Nov 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andreas SchulzeStephan Urban
Jun 24, 2010·Pathologie-biologie·Y XieM Zhao
Oct 9, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Min LianXiaofeng Zheng
Mar 25, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Matthias EngelkeStephan Urban
Nov 20, 2008·Cellular Microbiology·Corinna M BremerDieter Glebe
Dec 6, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anja MeierStephan Urban
Oct 14, 2011·Journal of Applied Microbiology·K W LeeW S Tan
Nov 30, 2004·Virus Research·Volker Bruss
Oct 16, 2004·Clinics in Liver Disease·Jia-Yee Lee, Stephen Locarnini
Jan 15, 2014·Current Opinion in Virology·Thomas F BaumertStephan Urban
Sep 1, 2009·Viruses·Stephanie Schädler, Eberhard Hildt
Sep 11, 2014·World Journal of Gastroenterology : WJG·Wen Siang Tan, Kok Lian Ho
May 15, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·S De FalcoG Fassina
Jul 1, 2020·Annual Review of Virology·Stefan SeitzRalf Bartenschlager
Feb 20, 2018·World Journal of Gastroenterology : WJG·Rosario CasillasFrancisco Rodríguez-Frías

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