PMID: 1413498Nov 1, 1992

Myxoma virus M11L ORF encodes a protein for which cell surface localization is critical in manifestation of viral virulence

Virology
K GrahamGrant McFadden

Abstract

Myxoma virus (MYX) induces extensive immunosuppression in infected rabbits and is associated with high levels of mortality. The virus encodes multiple gene products designed to circumvent the cellular immune response to the viral infection. Deletion analysis has shown that the M11L open reading frame (ORF) is an important virulence factor which downregulates leukocyte infiltration of MYX-induced tumors. To investigate the role of the M11L protein in viral pathogenesis, we sequenced the MYX M11L ORF and showed that the sequence has motifs consistent with a 166-aa class III membrane-spanning molecule possessing a single transmembrane helix near the C-terminus and a 142-aa N-terminal extracellular domain that has six cysteine residues plus two consensus N-glycosylation sites. Transcription analysis indicates that M11L is expressed as an early gene, and surface immunofluorescence studies with anti-M11L antibodies reveal that M11L protein is transported to the infected cell surface. Immunoprecipitation analysis of an attenuated viral recombinant, vMYX-GF-delta M11L, indicates that an M11L variant protein with an altered C-terminus is synthesized at about 45% of wild type levels; however, it is not detectable on the cell surface, sug...Continue Reading

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Citations

Feb 1, 1997·Journal of Neuroimmunology·Grant McFaddenL Sedger
Jun 6, 2002·Microbes and Infection·Malgorzata KrzyzowskaMarek Niemialtowski
May 3, 2000·The Journal of Experimental Medicine·Helen EverettGrant McFadden
Jun 26, 2002·Viral Immunology·Peter J Kerr, Grant McFadden
Jul 10, 1999·Immunological Reviews·P NashGrant McFadden
Jun 15, 2004·Journal of Virology·Gen WangGrant McFadden
Aug 6, 2002·Current Opinion in Microbiology·Helen Everett, Grant McFadden
Aug 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Denis R BurtonMarc Kvansakul
Apr 6, 2001·Seminars in Immunology·J L Shisler, B Moss
Nov 24, 1999·Virology·C CameronGrant McFadden
Dec 21, 2005·Virology·John M Taylor, Michele Barry
Jul 1, 2008·Advances in Virus Research·Steven J WerdenGrant McFadden
Apr 16, 2017·The Journal of Biological Chemistry·Mohd Ishtiaq AnasirMarc Kvansakul
Mar 11, 2015·Viruses·Peter J KerrEdward C Holmes
Oct 7, 2017·Viruses·Marc KvansakulMark G Hinds
Aug 9, 2017·Viruses·Daniel Brian NicholsJessica Cottrell
May 23, 2014·Journal of Virology·Stephanie CampbellMarc Kvansakul
Jan 6, 2017·Journal of Virology·Suresh BanjaraMarc Kvansakul
Feb 14, 2007·Veterinary Research·Marianne M StanfordGrant McFadden
Jan 23, 1999·International Reviews of Immunology·E M WallC Upton

Related Concepts

M11L protein, Myxoma virus
DNA, Viral
Myxoma virus
Precipitin Tests
Viral Proteins
Pathogenicity
Immunoblotting, Reverse
Restriction Mapping
Unassigned Reading Frames
Homologous Sequences, Amino Acid

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