N-3 polyunsaturated fatty acids ameliorate hepatic steatosis via the PPAR-α/CPT-1α pathway in a mouse model of parenteral nutrition

Biochemical and Biophysical Research Communications
Feng TianXinying Wang

Abstract

Parenteral nutrition (PN) is one of the basic therapies for patients with intestinal failure; however, hepatic steatosis associated with PN limits the long-term use of PN. N-3 polyunsaturated fatty acids (PUFAs) have been used to improve clinical outcomes of patients receiving PN; however, the mechanisms by which n-3 PUFAs ameliorate hepatic steatosis remain unclear. In the present study, C57BL/6J mice were randomly assigned to three treatment groups, namely, enteral nutrition (EN), n-3 PUFAs, and n-6 PUFAs. Additionally, MK 886 was used to inhibit PPAR-α. After 7 days of intervention, mice were sacrificed, and liver tissue and serum samples were collected. Results from liver weight and liver triglyceride measurements and Oil Red O staining showed that n-3 PUFAs significantly reduced the liver triglyceride levels. In addition, treatment with n-3 PUFAs resulted in a greater decrease in serum triglyceride and low-density lipoprotein cholesterol levels compared to n-6 PUFAs. The key enzymes involved in FA oxidation, namely, PPAR-α and CPT-1α, were significantly restored at both the mRNA and protein levels in the n-3 PUFAs group. However, the benefits of n-3 PUFAs in improving serum and liver TG levels were abolished when the PPAR-...Continue Reading

Citations

Jul 26, 2019·Alimentary Pharmacology & Therapeutics·Ashley BondSimon Lal
Jun 4, 2019·Archives of Physiology and Biochemistry·Meryem BerrichiNaim Akhtar Khan
Jul 6, 2020·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Jianbo YangXinying Wang
May 26, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T A Mariqueo, J Zúñiga-Hernández
Mar 24, 2021·Molecular Nutrition & Food Research·Gowtham SubramanianSelvakumar Subramaniam
Jul 23, 2021·JPEN. Journal of Parenteral and Enteral Nutrition·Shanshan ChenYing Wang

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