n-3 polyunsaturated fatty acids provoke a specific transcriptional profile in rabbit adipose-derived stem cells in vitro

Journal of Animal Physiology and Animal Nutrition
Eкaterina VackovaIvan Penchev Georgiev

Abstract

Adipose-derived stem cells (ADSCs) possess multipotent properties, and their proper functionality is essential for further development of metabolic disorders. In the current study, we explored the impact of two n-3 LC-PUFAs (long-chain polyunsaturated fatty acids, DHA-docosahexaenoic; C22:6, and EPA-eicosapentaenoic; C20:5) on a specific profile of lipolytic-related gene expressions in the in vitro-differentiated subcutaneous and visceral ADSCs from rabbits. The subcutaneous and visceral ADSCs were obtained from 28-day-old New Zealand rabbits. The primary cells were cultured up to passage 4 and were induced for adipogenic differentiation. Thereafter, the differentiated cells were treated with 100 µg EPA or DHA for 48 hr. The total mRNA was isolated and target genes expression evaluated by real-time RCR. The results demonstrated that treatment of rabbit ADSCs with n-3 PUFAs significantly enhanced mRNA expression of Perilipin A, while the upregulation of leptin and Rab18 genes was seen mainly in ADSCs from visceral adipose tissue. Moreover, the EPA significantly enhanced PEDF (Pigment Derived Epithelium Factor) mRNA expression only in visceral cells. Collectively, the results suggest activation of an additional lipolysis pathway ...Continue Reading

References

Dec 17, 1997·Biochemical and Biophysical Research Communications·G FrühbeckJ A Martínez
Aug 19, 2000·The Journal of Biological Chemistry·D L BrasaemleC Londos
Oct 30, 2001·The Proceedings of the Nutrition Society·P Trayhurn, J H Beattie
Jun 18, 2003·The Journal of Cell Biology·Carole SztalrydConstantine Londos
Jan 6, 2004·The Journal of Biological Chemistry·Naoto ArimuraRyuichiro Sato
May 18, 2004·Experimental Hematology·Darko BosnakovskiToru Fujinaga
Jun 8, 2004·The Journal of Clinical Endocrinology and Metabolism·Erin E Kershaw, Jeffrey S Flier
Jun 23, 2004·Laboratory Animals·Amalia E Yanni
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner
Jan 18, 2005·Cell and Tissue Research·Darko BosnakovskiToru Fujinaga
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Cullen M TaniguchiC Ronald Kahn
Nov 1, 2006·The Journal of Biological Chemistry·Martina SchweigerRobert Zimmermann
Feb 3, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Martin LaclaustraJosé M Ordovas
Feb 23, 2007·Annual Review of Nutrition·Robin E DuncanHei Sook Sul
Jun 12, 2008·Histochemistry and Cell Biology·Toyoshi FujimotoYuki Shinohara
Dec 5, 2008·Digestive Diseases and Sciences·Susan E WozniakEldo E Frezza
Jan 1, 2009·Molecular and Cellular Biochemistry·Dawn L BrasaemleAlexis Rothenberg
May 23, 2009·Journal of Cell Science·Krishnakant G SoniJuan S Bonifacino
Jul 9, 2009·Cell Metabolism·Seamus CroweMatthew J Watt
Jul 25, 2009·Molecular and Cellular Biochemistry·Yu-cheng LiGong-she Yang
Dec 23, 2009·The International Journal of Biochemistry & Cell Biology·Maryam AhmadianHei Sook Sul
Aug 25, 2010·Molecular and Cellular Biology·Sarah M ChoiMorris J Birnbaum
Aug 11, 2011·PloS One·Marina R PulidoMaria M Malagón
Aug 30, 2012·The Journal of Clinical Endocrinology and Metabolism·Kyle L SunderlandKevin R Short
Dec 26, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Amita YadavAnil Yadav
Dec 1, 2010·Journal of Ultrasound·L MoliniS Bianchi
Apr 9, 2013·Indian Journal of Endocrinology and Metabolism·Gilberto Paz-FilhoJulio Licinio
May 17, 2014·Journal of Cellular and Molecular Medicine·Cezary WójcikEduard Poels
Jan 6, 2016·Journal of Clinical Medicine·Marijana Todorčević, Leanne Hodson
May 6, 2016·Frontiers in Endocrinology·Sung Sik ChoeJae Bum Kim
May 14, 2016·In Vitro Cellular & Developmental Biology. Animal·Ekaterina VachkovaI Penchev Georgiev
Feb 27, 2017·Preventive Veterinary Medicine·Catalina PicassoAndres Perez

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