n-3 polyunsaturated fatty acids supplementation decreases asymmetric dimethyl arginine and arachidonate accumulation in aging spontaneously hypertensive rats

European Journal of Nutrition
Laura RaimondiAlessandro Mugelli

Abstract

Plasma accumulation of asymmetric dimethyl arginine (ADMA) is considered as a risk factor for endothelial dysfunction and a strong predictor for coronary heart diseases. Eicosapentaenoic (EPA) and docosahexaenoic (DHA) increasing plasma levels have been positively associated with reduced cardiovascular mortality with a mechanism( s) yet unclear. We hypothesised that ADMA reduction might be a part of EPA and DHA beneficial effects on the cardiovascular system. To verify this hypothesis we measured ADMA plasma levels in aged spontaneously hypertensive rats (SHR) supplemented for 8 weeks with EPA and DHA. 16-month-old SHR were supplemented with EPA and DHA (EPA-DHA) or with olive oil (1 g/kg/day; OLIVE). At the end of the treatments, the plasma of each animal was analysed for 1) the total fatty acid composition, by gas-cromatography, 2) ADMA levels, by high pressure liquid chromatography, 3) nitrite and homocysteine concentration by chemiluminescence and by polarisation immunoassay respectively. Moreover, the activity of dimethyl arginine dimethyl amino hydrolase, the main enzyme involved in ADMA metabolism, was measured spectrophotometrically in the kidney from each rat. Animals supplemented with EPA and DHA showed: 1) lower ADMA...Continue Reading

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Citations

Oct 8, 2008·Bioscience, Biotechnology, and Biochemistry·Phyllis S Y TamYoko Fujiwara
Jan 24, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Kazuo YamagataMotoki Tagami
Aug 25, 2015·BioMed Research International·Michela ZanettiRenzo Carretta
May 16, 2012·Free Radical Biology & Medicine·Pitchai Balakumar, Gaurav Taneja
May 15, 2016·Amino Acids·Dominique HermierFrançois Mariotti
Oct 4, 2016·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·G ColussiL A Sechi
Feb 12, 2011·American Journal of Hypertension·Yuichiro YanoKazuomi Kario
May 9, 2009·Journal of Agricultural and Food Chemistry·Zhen-Yu ChenYu Huang

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