N-3 PUFAs attenuate ischemia/reperfusion induced intestinal barrier injury by activating I-FABP-PPARγ pathway

Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
Xinying WangJieshou Li

Abstract

This study was designed to investigate whether n-3 PUFAs attenuate ischemia/reperfusion (I/R) induced intestinal barrier injury by activating I-FABP-PPARγ pathway. 24 Male Sprague-Dawley rats were assigned to 4 groups: control group, I/R group, pretreated with n-3 PUFAs for 7 days before I/R (group 3), pretreated with peroxisome proliferator-activated receptor (PPARγ) agonist 30 min before I/R (group 4). The serum and intestinal mucosa samples were collected. I/R disrupted the structure of intestinal tight junctions (TJs) and reduced occludin expression. The intestinal fatty acid binding protein (I-FABP) was elevated in plasma while decreased in cells. PPARγ expression in nucleus of intestinal mucosa was attenuated. N-3 PUFAs attenuated the damaged TJ structure and elevated occludin, intracellular I-FABP and PPARγ expression. A PPARγ agonist had the same effect as n-3 PUFAs. The intestinal barrier is severely damaged after I/R, which is related to the redistribution of I-FABP. Our findings firstly indicate that n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARγ pathway, and then upregulating TJ protein expression.

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Citations

Sep 10, 2014·Lipids in Health and Disease·Yang LiJieshou Li
Dec 21, 2013·The Journal of Nutritional Biochemistry·Wan ShenMichael K McIntosh
Aug 1, 2020·Nutritional Neuroscience·Sian EgertonCatherine Stanton
Mar 23, 2018·Digestive Diseases and Sciences·Shin NishiumiMasaru Yoshida
Sep 19, 2019·Molecular Nutrition & Food Research·Lifeng GuiZhiguo Liu
Dec 8, 2017·International Journal of Molecular Sciences·Lara CostantiniNicolò Merendino
Mar 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun WangGuosheng Wu

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