N 6 -methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus.

Virology Journal
Zhurui LiBaoan Song

Abstract

N 6 -methyl-adenosine (m6A) is a prevalent RNA modification in many species. Abnormal m6A methylation levels can lead to RNA dysfunction and can cause diseases. Tobacco mosaic virus (TMV) is one of the most devastating viruses for agricultural plants. It has many hosts, particularly including tobacco and other members the family Solanaceae. However, it remains unclear whether the abnormal growth induced by TMV is associated with the m6A level. A rapid and accurate analytical method using ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HR - MS/MS) was developed to analyse the adenosine (A), cytidine (C), guanosine (G), uridine (U), and m6A contents in the tobacco leaf, and the m6A/G ratio was used to evaluate the m6A level. Subsequent protein sequence alignments were used to find the potential methylases and demethylases in Nicotiana tabacum (N. tabacum). Finally, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was used to analyse the gene expression levels of the potential methylases and demethylases in the N. tabacum leaf. The results showed that TMV reduced the m6A level. Moreover, protein sequence alignments revealed partial homology a...Continue Reading

References

Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R DesrosiersF Rottman
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Keiko HoriuchiTatsuhiko Kodama
Oct 9, 2007·Electrophoresis·Michael G Cornelius, Heinz H Schmeiser
Jan 29, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yoshiyasu FukusumiMasahide Asano
Feb 17, 2009·Plant Physiology·Christina HofmannManfred Heinlein
Feb 24, 2009·Nature·Julia FischerUlrich Rüther
Apr 28, 2010·Nucleic Acids Research·Zsuzsanna BodiRupert G Fray
Nov 16, 2010·Nature Genetics·Chris ChurchRoger D Cox
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cintia F Hongay, Terry L Orr-Weaver
Oct 18, 2011·Nature Chemical Biology·Guifang JiaChuan He
Oct 25, 2011·Molecular Plant Pathology·Karen-Beth G ScholthofGary D Foster
May 12, 2012·Nature·Dan DominissiniGideon Rechavi
Oct 11, 2012·Journal of the American Chemical Society·Baoen ChenCai-Guang Yang
Mar 5, 2013·Genomics, Proteomics & Bioinformatics·Yamei NiuYun-Gui Yang
Dec 10, 2013·Nature Chemical Biology·Jianzhao LiuChuan He
Jan 29, 2014·Neuroscience and Biobehavioral Reviews·Erin L ZelinskiRobert J McDonald
Mar 13, 2014·The Journal of Biological Chemistry·Chong FengZhongzhou Chen
May 9, 2014·Nature Communications·Nicolas SierroNikolai V Ivanov
Nov 29, 2014·Nature Communications·Guan-Zheng LuoChuan He
Jun 13, 2015·Nucleic Acids Research·Xin DengChuan He

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Citations

Oct 29, 2019·The Plant Journal : for Cell and Molecular Biology·Omer MurikOren Ostersetzer-Biran
May 16, 2020·Frontiers in Cell and Developmental Biology·Hongxiang ZhengNa Sui
Jun 13, 2019·Cancer Science·Masamitsu KonnoHideshi Ishii
Jan 24, 2021·Journal of Experimental Botany·Jiaying ZhuXiuren Zhang
May 10, 2021·Current Opinion in Plant Biology·Yanlin ShaoHao Yu
Nov 13, 2020·Biotechnology Advances·Hong-Xiang ZhengNa Sui

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

AlignX
SPSS Statistics
BLAST
Vector NTI Suite

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