N-Acetyl-Cysteine and l-Carnitine Prevent Meiotic Oocyte Damage Induced by Follicular Fluid From Infertile Women With Mild Endometriosis

Reproductive Sciences
Vanessa S I GiorgiPaula Andrea Navarro

Abstract

This study evaluated the potential protective effect of the antioxidants, l-carnitine (LC) and N-acetyl-cysteine (NAC), in preventing meiotic oocyte damage induced by follicular fluid (FF) from infertile women with mild endometriosis (ME). We performed an experimental study. The FF samples were obtained from 22 infertile women undergoing stimulated cycles for intracytoplasmic sperm injection (11 with ME and 11 without endometriosis). Immature bovine oocytes were submitted to in vitro maturation (IVM) divided into 9 groups: no-FF (No-FF); with FF from control (CFF) or ME (EFF) groups; and with LC (C + LC and E + LC), NAC (C + NAC and E + NAC), or both antioxidants (C + 2Ao and E + 2Ao). After IVM, oocytes were immunostained for visualization of microtubules and chromatin by confocal microscopy. The percentage of meiotically normal metaphase II (MII) oocytes was significantly lower in the EFF group (51.35%) compared to No-FF (86.36%) and CFF (83.52%) groups. The E + NAC (62.22%), E + LC (80.61%), and E + 2Ao (61.40%) groups showed higher percentage of normal MII than EFF group. The E + LC group showed higher percentage of normal MII than E + NAC and E + 2Ao groups and a similar percentage to No-FF and CFF groups. Therefore, FF fr...Continue Reading

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Citations

Dec 22, 2015·Cell and Tissue Research·Michele Gomes Da Broi, Paula Andrea Navarro
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Jul 14, 2017·Journal of Ovarian Research·Cláudia FreitasHenrique Almeida
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Dec 29, 2020·Reproductive Biomedicine Online·Liliane Fabio Isidoro da SilvaPaula Andrea Navarro
Mar 20, 2021·Reproductive Biomedicine Online·Caroline M Da LuzPaula A Navarro

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Methods Mentioned

BETA
confocal microscopy
Confocal

Software Mentioned

SAS

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