PMID: 8596698Feb 1, 1996Paper

N-Acetyl-L-cysteine and its derivatives activate a Cl- conductance in epithelial cells

Pflügers Archiv : European journal of physiology
M KöttgenK Kunzelmann

Abstract

N-Acetyl-L-cysteine (NAC) is a widely used mucolytic drug in patients with a variety of respiratory disorders including cystic fibrosis (CF). The beneficial effects of NAC are empirical and the exact mechanism of action in the airways remains obscure. In the present study we examined the effects on whole-cell (wc) conductance (Gm) and voltage (Vm) of NAC and the congeners S-carboxymethyl-L-cysteine (CMC) and S-carbamyl-L-cysteine (CAC) and L-cysteine in normal and CF airway epithelial cells. L-Cysteine (1 mmol/l) had no detectable effect. The increase in Gm (delta Gm) by the other compounds was concentration dependent and was (all substances at 1 mmol/l) 3.8 +/- 1.4 nS (NAC; n = 11), 4.2 +/- 1.0 nS (CMC; n = 16) and 3.8 +/- 1.6 nS (CAC; n = 18), respectively. The changes in Gm were paralleled by an increased depolarization (delta Vm) when extracellular Cl- concentration was reduced to 34 mmol/l: under control conditions = -4.1 +/- 2.1 versus 10.2 +/- 2.1 mV in the presence of NAC, CMC, CAC (n = 36). In the presence of NAC, CMC and CAC, the reduction in Cl- concentration was paralleled by a reduction of Gm by 2.1 +/- 0.4 nS (n = 35), indicating that all substances acted by increasing the Cl- conductance. Analysis of intracellula...Continue Reading

References

Jan 1, 1992·Cancer Chemotherapy and Pharmacology·J A van LaarG J Peters
Jun 1, 1992·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Dec 1, 1990·The American Journal of Physiology·T JillingK L Kirk
Jan 1, 1989·Respiration; International Review of Thoracic Diseases·C MaayanS Godfrey
Jan 1, 1984·Annual Review of Physiology·B Sakmann, E Neher
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Oct 1, 1994·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Oct 1, 1994·Pflügers Archiv : European journal of physiology·T KoslowskyK Kunzelmann
Sep 1, 1994·Pflügers Archiv : European journal of physiology·M KöttgenR Greger

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Citations

Nov 12, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·F GuizzardiG Meyer
May 24, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E DoussetR Guieu
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Horst FischerBeate Illek
Jun 21, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dong-Ho OhSangkil Lee
Dec 2, 2009·Cell Biology International·Georgia VarelogianniMarie Johannesson
Feb 28, 2002·Biophysical Journal·Melissa A Harrington, Ron R Kopito
Sep 24, 2004·The Journal of Membrane Biology·R SchreiberK Kunzelmann
Jul 29, 2004·Journal of Cellular Physiology·Thierry RochatLan Jornot
Dec 12, 2000·The Journal of Biological Chemistry·J V WuJ J Wine
Mar 29, 2001·The Journal of Biological Chemistry·G LambertK Kunzelmann
Mar 5, 1998·The American Journal of Physiology·M M CloutierL Guernsey
Oct 7, 2018·Pflügers Archiv : European journal of physiology·Yukiko IkeuchiTakashi Nakahari
May 29, 2021·The Journal of Experimental Biology·Varshinie PillaiEbrahim Lari

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