N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Jitender Kumar Bhardwaj, Priyanka Saraf

Abstract

Methoxychlor (MXC), an organo-chlorine insecticide, is a reproductive toxicant in females, causing apoptosis-mediated follicular atresia. To elucidate the potentials of Methoxychlor as a geno-toxicant, granulosa cells of healthy antral follicles, exposed to MXC and antioxidant, N-acetyl-l-cysteine, were studied by the terminal deoxynucleotidyltransferase-dUTP nick end-labelling and single-cell gel electrophoresis (comet) assays. MXC caused DNA fragmentation, as revealed by the increased incidence of dark brown condensed TUNEL positive cells in contrast with lightly brown TUNEL negative cells with maximum TUNEL positive cells were observed in 100 μg/mL MXC treated groups. Quantitatively, maximum geno-toxicity was exhibited at highest MXC treatment with percent tail DNA as 17.87 ± 0.85, 41.16 ± 3.94, and 47.73 ± 3.71 in comparison with control (0.65 ± 0.03, 2.91 ± 0.27, and 7.16 ± 1.39) after 24, 48 and 72 h exposure duration, respectively. MXC treated groups exhibited Type 1-Type 3 comets as compared to Type 0 comets in control groups. Supplementation of NAC led to significant (p < 0.05) decline in geno-toxicity in MXC treated groups with maximum amelioration observed at 5 and 10 mM. Consequently, increased DNA damage attributed...Continue Reading

Citations

Sep 17, 2020·Reproductive Sciences·Jitender Kumar BhardwajPriyanka Saraf
Mar 13, 2021·Journal of Biochemical and Molecular Toxicology·Elçin BakırÜlkü Ündeğer Bucurgat
May 30, 2021·Journal of Biochemical and Molecular Toxicology·Jitender K BhardwajPriyanka Saraf
Jun 1, 2021·Chemico-biological Interactions·Sharmistha ChoudhuriShailendra Asthana
Sep 10, 2021·Reproductive Biology and Endocrinology : RB&E·Yang MuJing Yang
Sep 17, 2021·Environmental Toxicology·Jisheng NieQunwei Zhang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis