N-acetyl-L-cysteine pre-treatment protects cryopreserved bovine spermatozoa from reactive oxygen species without compromising the in vitro developmental potential of intracytoplasmic sperm injection embryos

Andrologia
L PérezR Felmer

Abstract

Excess of reactive oxygen species (ROS) on in vitro embryo production systems negatively affects the quality and developmental potential of embryos, as result of a decreased sperm quality and increased DNA fragmentation. This issue is of major importance in assisted fertilisation procedures such as intracytoplasmic sperm injection (ICSI), because this technique does not allow the natural selection of competent spermatozoa, and therefore, DNA-damaged spermatozoa might be used to fertilise an egg. The aim of this study was to investigate a new strategy to prevent the potential deleterious effect of ROS on cryopreserved bovine spermatozoa. We evaluated the effect of a sperm pre-treatment with different concentrations of N-acetyl-L-cysteine (NAC) on ROS production, viability and DNA fragmentation and assessed the effect of this treatment on the in vitro developmental potential and quality of embryos generated by ICSI. The results show a strong scavenging effect of 1 and 10 mm NAC after exposure of spermatozoa to a ROS inducer, without compromising the viability and DNA integrity. Importantly, in vitro developmental potential and quality of embryos generated by ICSI with spermatozoa treated with NAC were not affected, confirming the...Continue Reading

References

May 26, 1999·Journal of Reproduction and Fertility·R J Aitken
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Citations

Jul 11, 2018·PloS One·Adriano Felipe Perez SiqueiraMayra Elena Ortiz D'Ávila Assumpção
Jan 25, 2019·Climacteric : the Journal of the International Menopause Society·Y LiA O Mueck
Dec 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·María José ContrerasRicardo Felmer
Apr 10, 2019·Antioxidants·David Martin-HidalgoMarco G Alves
Sep 20, 2016·Critical Reviews in Toxicology·Xu WangZonghui Yuan
Aug 5, 2021·Environmental Science and Pollution Research International·Mohamed A AwadMoukhtar H G Moussa

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