N-Acetylcysteine enhances the lung cancer inhibitory effect of epigallocatechin-3-gallate and forms a new adduct.

Free Radical Biology & Medicine
Joshua D LambertChung S Yang

Abstract

The major tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG), inhibits carcinogenesis in many in vivo models. Many potential mechanisms of action have been proposed based on cell line studies, including prooxidant activity. In the present study, we studied the effect of N-acetylcysteine (NAC) on the inhibitory effects of EGCG on lung cancer cell growth. We found that NAC (0-2 mM) dose dependently enhanced the growth inhibitory activity of EGCG against murine and human lung cancer cells. The combination of NAC and EGCG caused an 8.8-fold increase in apoptosis in CL13 mouse lung cancer cells compared to treatment with either agent alone. Addition of 2 mM NAC increased the stability of EGCG in the presence of CL13 cells (t 1/2=8.5 h vs 22.7 h). Intracellular levels of EGCG were increased 5.5-fold by the addition of 2 mM NAC. HPLC and LC-MS analyses of cell culture medium from CL13 cells treated with EGCG and NAC for 24 h revealed that EGCG-2'-NAC was time dependently formed. This adduct was not formed in the absence of NAC. The present results show that under cell culture conditions, EGCG and NAC interact to form a previously unreported adduct, EGCG-2'-NAC, which may contribute to enhancement of EGCG-mediated cell killing.

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Citations

Nov 27, 2014·Journal of Experimental & Clinical Cancer Research : CR·Afshin AminiDavid Lawson Morris
Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Taiki MoriTsutomu Nakayama
Sep 21, 2016·Critical Reviews in Food Science and Nutrition·Ren-You GanHarold Corke
Mar 24, 2018·Nutrition and Cancer·Sunil Kumar DhatwaliaDevinder K Dhawan
Sep 22, 2019·International Journal of Molecular Sciences·Itika AroraTrygve O Tollefsbol
Dec 28, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hsiao-Hua ChangJiiang-Huei Jeng
Apr 26, 2012·Integrative Cancer Therapies·Heidi FritzDean Fergusson
Jul 6, 2021·Journal of Nutrition and Metabolism·Gerry K Schwalfenberg
Aug 5, 2021·Journal of Agricultural and Food Chemistry·Yuki TakashimaShoko Kobayashi

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