N-acetylcysteine prevents rotenone-induced Parkinson's disease in rat: An investigation into the interaction of parkin and Drp1 proteins

Brain Research Bulletin
Arman RahimmiKambiz Hassanzadeh

Abstract

There are convincing evidences that oxidative stress has an important role in both the initiation and progression of Parkinson's disease. N-acetylcysteine (NAC) is shown to have antioxidant properties via fortifying glutathione which is one of the main endogenous antioxidant systems. Therefore our study was aimed to evaluate the effect of NAC in management of Parkinson's disease. To this aim, male Wistar rats (10-12 months) received rotenone 2.5mg/kg/48 h intraperitoneally (ip) to induce a Parkinson's disease model. Pretreatment with NAC (25 and 50mg/kg/48 h ip) was administered 1h before the rotenone injection. Three behavioral tests (rotarod, rearing and bar tests) were performed for motor function assessment. Dopamine levels of dopaminergic areas in rat brain including substantia nigra (SN) and striatum (ST) were assessed using high performance liquid chromatography analysis to measure the loss of dopamine. Western blot analysis was also done for parkin and Drp1 (dynamin related protein-1) proteins quantification in SN and ST. Our results indicated that NAC significantly ameliorated the rotenone-induced motor dysfunction and dopamine loss. Furthermore, NAC was able to prevent the rotenone-induced changes in parkin and Drp1 l...Continue Reading

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Citations

Aug 15, 2015·Neurochemistry International·Debashis Dutta, Kochupurackal P Mohanakumar
Aug 27, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nuramatjan AblatXiaoping Pu
Jul 24, 2018·Alcoholism, Clinical and Experimental Research·Maria Elena QuintanillaYedy Israel
Sep 4, 2015·Molecular and Cellular Biochemistry·Ana-Maria Enciu, Laurentiu M Popescu
Dec 10, 2019·Oxidative Medicine and Cellular Longevity·Arman RahimmiKambiz Hassanzadeh
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Aug 25, 2020·Oxidative Medicine and Cellular Longevity·Alejandra Guillermina Miranda-DíazErnesto Germán Cardona-Muñoz
May 30, 2021·Brain Research Bulletin·Kambiz HassanzadehMarco Feligioni
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Aug 27, 2021·Metabolic Brain Disease·Wachiryah Thong-AsaSuchawalee Jutarattananon
Mar 20, 2018·Neurochemistry International·Navneet Ammal Kaidery, Bobby Thomas

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