PMID: 9523930Apr 2, 1998Paper

N-acetylcysteine reduces methemoglobin in an in-vitro model of glucose-6-phosphate dehydrogenase deficiency

Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine
R O WrightB Magnani

Abstract

To determine whether N-acetylcysteine (NAC) reduces methemoglobin (MHB) in an in-vitro model of glucose-6-phosphate dehydrogenase (G6PD) deficiency, given that methylene blue is an ineffective MHB antidote in G6PD deficiency. Five volunteers donated blood, which was divided equally into 2 test tubes, centrifuged, and washed with Tris-Mopps buffer (pH 7.4, 15 mmol/L glucose). Both tubes were incubated with epiandrosterone (EA) (400 micromol), a specific inhibitor of G6PD. After 75 microL of 0.18 mol hydroxylamine (HA) was added to induce MHB formation, 150 microL of NAC (20 mg/mL) was added to tube 1 and 150 microL of phosphate-buffered saline (PBS) was added to tube 2 as a volume control. Serial MHB levels are reported as a percentage of total hemoglobin (Hb). G6PD activity was measured at baseline, 15 minutes after EA, and at 5 hours. Mean G6PD activity at baseline was 9.2+/-2.9 U/g Hb (normal >4.6 U/g Hb); 15 minutes after EA was 3.0+/-1.0 U/g Hb; and at experiment's end was 2.3+/-0.7 U/g Hb. The mean (+/-SD) areas under the concentration-time curves (AUCs) of NAC-EA-HA and PBS-EA-HA samples were compared using an unpaired t-test and were significantly different: PBS-EA-HA, 20,400+/-1,100 % min, vs NAC-EA-HA, 10,400+/-1,000 %...Continue Reading

References

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Citations

Sep 30, 2008·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·You Hwan JoGil Joon Suh
Jul 23, 2015·Case Reports in Emergency Medicine·Birdal YildirimŞükrü Kasap
Feb 15, 2000·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·P A ChykaM I Herman
Jul 4, 2003·American Journal of Physiology. Cell Physiology·Xueying Liu, Zoltán Spolarics
Jul 20, 2020·The Journal of Emergency Medicine·Rawan M Eskandarani, Faisal S Alghamdi
Oct 26, 1999·Annals of Emergency Medicine·R O WrightA D Woolf

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