N-acetylcysteine relieves oxidative stress and protects hippocampus of rat from radiation-induced apoptosis by inhibiting caspase-3

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jianguo LiQiang Liu

Abstract

It has been recognized that radiation-induced effects remain a significant risk. An accumulation of reactive oxygen species (ROS) is considered to be one factor that contributes to neurodegenerative changes. The aim of our study was to investigate the potential radioprotective effects of NAC. Male Sprague-Dawley rats underwent radiation. Irradiation was performed at room temperature with a 4-Gy dose of radiation. A dose of N-acetylcysteine (NAC) was performed 15 min prior to irradiation intraperitoneally. The methods of immunohistochemistry, TUNEL staining, Nissl staining, qRT-PCR, analysis of reactive oxygen species and Western blot were performed. In conclusion, our results demonstrate that NAC inhibits apoptosis induced by irradiation via the inhibition of caspase-3. We demonstrated a decrease in caspase-3 mRNA that was present at 24h of NAC treatment. Such mRNA decrease was accompanied by a decrease of protein. In the present study, NAC effectively antagonized oxidation induced by irradiation. These results provide evidence that the neural protective effect and the antioxidant effect of NAC contribute to metabolic activity.

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Citations

Jan 13, 2018·Journal of Radiation Research·Wei LongDeguan Li
Sep 6, 2018·Neural Computation·Beatrice Alexandra Golomb
Apr 24, 2020·Journal of Cellular Physiology·Elham MotallebzadehMehran Mohseni
Jun 8, 2017·Frontiers in Oncology·Osama Muhammad MariaThierry Muanza
Aug 9, 2019·Environmental Science and Pollution Research International·Tolga MercantepeFiliz Mercantepe
Apr 1, 2020·Histochemistry and Cell Biology·Sherif Mohamed ZakiFatma M Ghoneim
Jan 16, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel GonzalezMiroslav Strnad

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