N-acetyldopamine quinone methide/1,2-dehydro-N-acetyl dopamine tautomerase. A new enzyme involved in sclerotization of insect cuticle

FEBS Letters
S J Saul, M Sugumaran

Abstract

The enzyme system causing the side chain desaturation of the sclerotizing precursor, N-acetyldopamine (NADA), was solubilized from the larval cuticle of Sarcophaga bullata and resolved into three components. The first enzyme, phenoloxidase, catalyzed conversion of NADA to NADA quinone and provided it for the second enzyme (NADA quinone isomerase), which makes the highly unstable NADA quinone methide. Quinone methide was hydrated rapidly and nonenzymatically to form N-acetylnorepinephrine. In addition, it also served as the substrate for the last enzyme, quinone methide tautomerase, which converted it to 1,2-dehydro-NADA. Reconstitution of NADA side chain desaturase activity was achieved by mixing the last enzyme fraction with NADA quinone isomerase, obtained from the hemolymph of the same organism, and mushroom tyrosinase. Therefore, NADA side chain desaturation observed in insects is caused by the combined action of three enzymes rather than the action of a single specific NADA desaturase, as previously thought.

Citations

Jan 1, 1990·Archives of Insect Biochemistry and Physiology·M SugumaranH Dali
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·M Sugumaran, K Nellaiappan
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·M SugumaranH Dali
Jan 1, 1992·Archives of Insect Biochemistry and Physiology·M SugumaranV Semensi
Jul 19, 2000·Archives of Biochemistry and Biophysics·M SugumaranK Valivittan
Feb 19, 2000·Biochemical and Biophysical Research Communications·M Sugumaran, K Nellaiappan
Feb 1, 1993·Chemico-biological Interactions·D C ThompsonP Moldéus
Jan 9, 1992·Biochimica Et Biophysica Acta·S O AndersenP Roepstorff
Sep 28, 1994·Biochimica Et Biophysica Acta·G B Wybrandt, S O Andersen
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M HallJ H Law
Feb 23, 2011·Marine Drugs·Manickam Sugumaran, William E Robinson
Jul 19, 2000·Insect Biochemistry and Molecular Biology·M R ChaseM Sugumaran
Sep 23, 2016·International Journal of Molecular Sciences·Manickam Sugumaran
Oct 25, 2016·International Journal of Molecular Sciences·Manickam Sugumaran, Hanine Barek
May 31, 2017·Rapid Communications in Mass Spectrometry : RCM·Hanine BarekManickam Sugumaran
Jan 1, 1995·Archives of Insect Biochemistry and Physiology·M Sugumaran, D Ricketts
Nov 26, 2009·Insect Biochemistry and Molecular Biology·Svend Olav Andersen

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