N-Acetylglucosamine 6-O-sulfotransferase-1 is required for brain keratan sulfate biosynthesis and glial scar formation after brain injury

Glycobiology
Haoqian ZhangK Kadomatsu

Abstract

Keratan sulfate (KS) is a glycosaminoglycan composed of repeating disaccharide units with sulfate residues at the C6 positions of galactose and N-acetylglucosamine (GlcNAc). The N-acetylglucosamine 6-O-sulfotransferase(s) (GlcNAc6ST) involved in the synthesis of KS in the central nervous system (CNS) has long been unidentified. Here, we report that a deficiency of GlcNAc6ST-1 leads to loss of 5D4-reactive brain KS and reduction of glial scar formation after cortical stab injury in mice. During the development of mice deficient in GlcNAc6ST-1, KS expression in the brain was barely detectable with the KS-specific antibody 5D4. The reactivity of 5D4 antibody with protein tyrosine phosphatase zeta (PTPzeta), a KS proteoglycan (KSPG), was abolished in the deficient mice. In adults, brain injury induced 5D4-reactive KS synthesis in the wounded area in wild-type (WT) mice but not in the deficient mice. Glial scar is formed via the accumulation of reactive astrocytes and is a major obstacle to axonal regeneration by injured neurons. Reactive astrocytes appeared to similar extents in the two genotypes, but they accumulated in the wounded area to a lesser extent in the deficient mice. Consequently, the deficient mice exhibited a marked r...Continue Reading

References

Nov 9, 2000·Nature·P J Horner, F H Gage
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
Nov 9, 2002·Science·Kevin Fox, Bruce Caterson
Feb 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Barbara Grimpe, Jerry Silver

❮ Previous
Next ❯

Citations

Jun 30, 2006·Annual Review of Cell and Developmental Biology·Hannes E Bülow, Oliver Hobert
Jul 5, 2013·PloS One·Kenichi HiranoKenji Kadomatsu
Mar 28, 2012·Expert Review of Neurotherapeutics·Bharath WootlaMoses Rodriguez
Mar 11, 2008·Biochemical and Biophysical Research Communications·Yukinari KatoHisashi Narimatsu
Aug 19, 2015·Experimental Neurology·Patrice D SmithJames W Fawcett
Sep 16, 2015·The European Journal of Neuroscience·Hisataka FujimotoShozo Jinno
May 26, 2012·The Journal of Comparative Neurology·Jae-Hyuk YiHerbert M Geller
Apr 3, 2012·Biochemical and Biophysical Research Communications·Michelle KilcoyneSiobhán S McMahon
Jan 24, 2008·Biochemical and Biophysical Research Communications·Norihito HayatsuHisashi Narimatsu
Oct 26, 2013·Neuroscience Research·Kenji Kadomatsu, Kazuma Sakamoto
Jan 13, 2015·Experimental and Therapeutic Medicine·Wen ZengMeng Mao
Jun 1, 2011·Biochemical and Biophysical Research Communications·Masatoshi HayashiNaoki Ishiguro
Jun 22, 2014·Archives of Biochemistry and Biophysics·Kenji Kadomatsu, Kazuma Sakamoto
Apr 13, 2017·Glycoconjugate Journal·Kazuchika Nishitsuji, Kenji Uchimura
Oct 25, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hitomi HoshinoKenji Uchimura
Dec 25, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Congxiao GaoNaoyuki Taniguchi
Apr 6, 2018·Molecular Neurodegeneration·Bevan S MainMark P Burns
Aug 3, 2019·The EMBO Journal·Vassilis StratouliasBertrand Joseph
Jan 19, 2018·Journal of Neuroscience Research·Naijil George, Herbert M Geller
Sep 6, 2015·Journal of Neuroscience Research·Rino UenoSusumu Kusunoki
Jul 20, 2018·Experimental Neurobiology·Heejung ChunC Justin Lee
Aug 14, 2020·Neural Regeneration Research·Amanda Phuong Tran, Jerry Silver
Jun 8, 2017·Frontiers in Molecular Neuroscience·Odile BartholomeBernard Rogister
Aug 18, 2018·The Biochemical Journal·Anthony J Hayes, James Melrose
Apr 4, 2021·Biomolecules·Aikaterini BerdiakiDragana Nikitovic
Nov 19, 2017·Trends in Neurosciences·Panpan YuHerbert M Geller
Jun 3, 2021·International Journal of Molecular Sciences·Kazuma SakamotoKenji Kadomatsu
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Claudia DesoleTiziana Crepaldi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Zenya ItoK Kadomatsu
Annals of the New York Academy of Sciences
Haoqian ZhangK Kadomatsu
© 2022 Meta ULC. All rights reserved