N-Acetylglucosaminyltransferase V exacerbates murine colitis with macrophage dysfunction and enhances colitic tumorigenesis

Journal of Gastroenterology
Shinichiro ShinzakiEiji Miyoshi

Abstract

Oligosaccharide structures and their alterations have important roles in modulating intestinal inflammation. N-Acetylglucosaminyltransferase V (GnT-V) is involved in the biosynthesis of N-acetylglucosamine (GlcNAc) by β1,6-branching on N-glycans and is induced in various pathologic processes, such as inflammation and regeneration. GnT-V alters host immune responses by inhibiting the functions of CD4(+) T cells and macrophages. The present study aimed to clarify the role of GnT-V in intestinal inflammation using GnT-V transgenic mice. Colitis severity was compared between GnT-V transgenic mice and wild-type mice. β1,6-GlcNAc levels were investigated by phytohemagglutinin-L4 lectin blotting and flow cytometry. We investigated phagocytosis of macrophages by measuring the number of peritoneal-macrophage-ingested fluorescent latex beads by flow cytometry. Cytokine production in the culture supernatant of mononuclear cells from the spleen, mesenteric lymph nodes, and bone-marrow-derived macrophages was determined by enzyme-linked immunosorbent assay. Clodronate liposomes were intravenously injected to deplete macrophages in vivo. Chronic-colitis-associated tumorigenesis was assessed after 9 months of repeated administration of dextra...Continue Reading

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Citations

Dec 26, 2015·Journal of Gastroenterology·Motohiro Nonaka
Mar 25, 2017·Journal of Biochemistry·Shinichiro ShinzakiEiji Miyoshi
May 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ana M DiasSalomé S Pinho
Dec 6, 2016·Frontiers in Microbiology·Rahul MittalXue Z Liu
Mar 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Depeng YangYu Li

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