N-acetylglucosaminyltransferase V inhibits the invasion of trophoblast cells by attenuating MMP2/9 activity in early human pregnancy

Placenta
Qinyin DengHongbo Qi

Abstract

The invasion and migration of trophoblast cells are essential steps of normal placentation and successful pregnancy. The process is well-regulated by many factors at the fetal-maternal surface. Inadequate invasion by trophoblast cells may lead to poor perfusion of the placenta or complications such as preeclampsia (PE). Accumulating evidence suggests that N-acetylglucosaminyltransferase V (MGAT5) is correlated with tumor invasion and metastasis. Our objective was to characterize MGAT5 expression and function during placental development. The expression of MGAT5 in humans in placental tissue from the first trimester was determined by immunohistochemistry. To investigate whether MGAT5 regulates trophoblast invasion and migration, we investigated invasion/migration of the HTR8/SVneo trophoblast cells and used human villous explants. Cell proliferation and apoptosis were measured by CCK-8 assay and flow cytometry, respectively. The activity of matrix metalloproteinase (MMP) 2/9, and the expression of tissue inhibitors of metalloproteinases (TIMPs) 1/2 were determined by gelatin zymography and Western blot, respectively. MGAT5 was specifically localized within the cytotrophoblast, the syncytiotrophoblast and the trophoblast columns ...Continue Reading

References

Oct 30, 1992·Cell·S Strickland, W G Richards
Mar 1, 1993·The Journal of Clinical Investigation·Y ZhouS J Fisher
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Senitiroh Hakomori
Apr 5, 2003·Annual Review of Biochemistry·John B Lowe, Jamey D Marth
Mar 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hirotoshi Dosaka-AkitaNaoyuki Taniguchi
Sep 17, 2004·The Journal of Clinical Investigation·Kristy Red-HorseSusan J Fisher
Oct 7, 2004·Glycoconjugate Journal·Jianguo Gu, Naoyuki Taniguchi
Apr 6, 2005·Biochemical and Biophysical Research Communications·Mari TomiieYuji Murata
Jul 23, 2005·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Deborah NewbyFiona Lyall
Oct 27, 2005·Placenta·M CohenP Bischof
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hirofumi IshimuraChikara Ohyama
Oct 16, 2007·Gynecologic and Obstetric Investigation·Marie Cohen, Paul Bischof
Oct 31, 2009·The International Journal of Developmental Biology·Martin Knöfler
Jul 6, 2010·Lancet·Eric A P SteegersRobert Pijnenborg
Jan 30, 2013·Biochemical and Biophysical Research Communications·Ju Hee LeeJeong-Heon Ko
Jun 29, 2013·Cellular and Molecular Life Sciences : CMLS·Melinda HalaszJulia Szekeres-Bartho
Jan 25, 2014·Cell Death & Disease·W LiH Wang

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Citations

Jan 6, 2017·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Margarita L Martinez-FierroJose M Ortiz-Rodriguez
Jun 24, 2016·Reproductive Sciences·Qinyin DengHongbo Qi
Jan 9, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Haiying RaoHongbo Qi
Feb 23, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Qinyin DengHongbo Qi
Sep 6, 2020·Journal of Cellular and Molecular Medicine·Balsam RizeqAllal Ouhtit
Mar 31, 2020·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Jing TianMark D Kilby

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