Abstract
N-acetylphytosphingosine (NAPS), a sphingolipid derivative, is one of the well-known signal molecules that mediates various cellular functions, including cell growth, differentiation, and apoptosis. In this study, we demonstrated that NAPS induces apoptosis of Jurkat cells by activating Bak, but not Bax, which are both members of a proapoptotic subfamily of the Bcl-2 proteins. NAPS activated caspase-8 in a FADD-independent manner, but the lack of caspase-8 did not suppress the activation of caspase-3 and -9 and cell death, indicating that caspase-8 activation does not play an important role in NAPS-induced cell death. The overexpression of Bcl-xL, an anti-apoptotic protein, completely inhibited the activation of the caspases and apoptosis, assuming that NAPS-induced apoptosis was initiated by the mitochondria. The expression levels of pro- and anti-apoptotic Bcl-2 family members were not changed by the NAPS treatment. However, Bad was translocated from the cytosol into the mitochondria, where it bound to Bcl-xL, and Bak was dissociated from Bcl-xL and conformationally changed. Taken together, these findings indicate that NAPS induced apoptosis of Jurkat cells in a mitochondria-dependent manner that was controlled by the translo...Continue Reading
References
Jan 1, 1991·Dermatologica·N Y SchürerP M Elias
May 2, 1998·The EMBO Journal·C ScaffidiM E Peter
Mar 23, 1999·The Journal of Cell Biology·G J GriffithsJ A Hickman
Apr 5, 2000·The Journal of Biological Chemistry·O CuvillierS Spiegel
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·A GewiesM B Cohen
Jun 6, 2000·The Journal of Biological Chemistry·Y TanM J Comb
Jul 27, 2000·Journal of Cellular Physiology·R N KolesnickA Alonso
Mar 30, 2001·The Journal of Biological Chemistry·E C Thornborrow, J J Manfredi
Apr 18, 2001·Molecular Pharmacology·O HwangJ D Lee
Apr 28, 2001·Science·M C WeiS J Korsmeyer
Jun 19, 2001·Genes & Development·W X ZongC B Thompson
Jun 22, 2001·FEBS Letters·J S LeeN J Cho
Jul 12, 2001·The Journal of Biological Chemistry·G Q WangH Rabinowich
Sep 22, 2001·FEBS Letters·H J KimM Y Kim
Nov 7, 2001·The Journal of Experimental Medicine·G Q WangH Rabinowich
Dec 26, 2001·Oncogene·G J GriffithsC Dive
Apr 27, 2002·Experimental Biology and Medicine·Eun Hyun Ahn, Joseph J Schroeder
May 31, 2002·Oncogene·Clarissa von HaefenPeter T Daniel
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Roberta A Gottlieb, David J Granville
Sep 21, 2002·Cancer Cell·Anthony LetaiStanley J Korsmeyer
Sep 25, 2002·Biochemical and Biophysical Research Communications·Mari Rios CandeloreSuzanne M Mandala
Nov 20, 2002·Biochemical and Biophysical Research Communications·Vilen A MovsesyanAlan I Faden
Jan 4, 2003·The Journal of Biological Chemistry·Kazuya IwaiToshiro Okazaki
Feb 1, 2003·The Biochemical Journal·Norman S Radin
Mar 1, 2003·Cancer Letters·Brad L UphamJames E Trosko
Mar 7, 2003·Il Farmaco·Marco MacchiaRomano Danesi
Apr 18, 2003·Cell Death and Differentiation·K KimuraE P Gelmann
May 6, 2003·Biochemical and Biophysical Research Communications·Luca Scorrano, Stanley J Korsmeyer
May 6, 2003·Biochimica Et Biophysica Acta·Jennifer A Shabbits, Lawrence D Mayer
Jun 6, 2003·Bioorganic & Medicinal Chemistry·Keiji ShikataTaro Tachibana
Oct 3, 2003·The Journal of Biological Chemistry·Moon-Taek ParkSu-Jae Lee
Jan 8, 2004·The Journal of Investigative Dermatology·Ho Jin KimTae-Yoon Kim
Jul 21, 2004·The Journal of Biological Chemistry·Chiou-Feng LinYee-Shin Lin
Citations
Mar 17, 2006·Acta Pharmacologica Sinica·Guan-Lei WangYong-Yuan Guan
May 28, 2010·Journal of Genetics·Subbashree Aparajita, Gyana Ranjan Rout
May 13, 2009·Biochemical Pharmacology·Yahya SalmaNathalie Andrieu-Abadie
Jul 30, 2011·JPEN. Journal of Parenteral and Enteral Nutrition·Jian ChenLi-ming Zhou
Jan 21, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Cheryl A DyerLoretta P Mayer