N-acylphosphatidylethanolamine synthesis in plants: occurrence, molecular composition, and phospholipid origin

Archives of Biochemistry and Biophysics
Kent D Chapman, T S Moore

Abstract

We have identified a microsomal phospholipid as N-acylphosphatidylethanolamine (NAPE) that was radiolabeled following incubation of 1-day-old cotyledons of cotton seedlings with [14C]ethanolamine. Radiolabeled NAPE comigrated with commercially available L-alpha-dipalmitoyl phosphatidyl(N-palmitoyl)ethanolamine (std-NAPE) in one- and two-dimensional TLC. This NAPE comprised 7.2 +/- 1.0% (by weight) of microsomal phospholipids when hot isopropanol was used to inactivate endogenous phospholipases prior to extraction of lipids. In vitro degradation of putative cottonseed radiolabeled NAPE by Streptomyces chromofuscus phospholipase D resulted in production of a ninhydrin-reactive, radiolabeled lipid which comigrated with N-acylethanolamine (NAE) that was produced from a similar enzymatic cleavage of std-NAPE. Transmethylation of cottonseed radiolabeled NAE yielded radiolabeled ethanolamine and fatty acid methyl esters, nearly all of which were saturated (myristate, palmitate, and stearate together were 92% of the acyl components of cottonseed NAE). Positional analysis and relative abundance of the O-acyl groups of cottonseed microsomal NAPE were determined following a double enzymatic cleavage with Trimeresurus flavoviridis venom (p...Continue Reading

Citations

Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher N JamesKent D Chapman
May 19, 2009·The Journal of Biological Chemistry·Lionel FaureJean-Jacques Bessoule
Aug 2, 2011·The Journal of Biological Chemistry·Evgeny Bulat, Teresa A Garrett
Feb 18, 2012·The Plant Cell·Patrick J HornKent D Chapman
Dec 27, 2013·International Journal of Endocrinology·Jordyn M StuartHeather B Bradshaw
Jun 1, 2006·The Journal of Cell Biology·Derk BinnsJoel M Goodman
Jan 31, 2012·Plant Science : an International Journal of Experimental Plant Biology·Denis CoulonJean-Jacques Bessoule
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Young Jin LeeBasil J Nikolau
Aug 18, 2012·Plant Signaling & Behavior·Aruna Kilaru, Kent D Chapman
Dec 30, 2014·Molecular Biology of the Cell·Bethany R CartwrightJoel M Goodman
Mar 23, 2000·European Journal of Biochemistry·M Van Der SteltJ F Vliegenthart
Jan 11, 2007·Journal of Lipid Research·René BartzKent D Chapman
Aug 23, 2007·Chemistry & Biodiversity·Aruna KilaruKent D Chapman
Jan 9, 2014·The Plant Journal : for Cell and Molecular Biology·Elison B BlancaflorKent D Chapman
Feb 23, 2020·Frontiers in Plant Science·Shaoping LuLiang Guo
Jun 11, 1999·Plant Physiology·P E PfefferY Shachar-Hill
May 24, 1996·Chemistry and Physics of Lipids·H H SchmidV Natarajan
Oct 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A FontanaD Piomelli
May 9, 2014·The Journal of Physical Chemistry. a·Emily S MohnJohn J Breen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.