N-acylphosphatidylethanolamines: effect of the N-acyl chain length on its orientation

Biophysical Journal
C P LafranceM Pézolet

Abstract

N-Acylphosphatidylethanolamines, or NAPEs, are found in tissues involved in degenerating processes, such as dehydrated endosperm of seeds, erythrocyte membranes, or cell injury. To determine the conformation and orientation of the acyl chains of these phospholipids, NAPEs with deuterated N-acyl chains of 6 and 16 carbon atoms were synthesized and studied by transmission and attenuated total reflectance (ATR) infrared spectroscopy. For N-C16d-DPPE, the ATR measurements show that the N-acyl chain has the same orientation as the two acyl chains attached to the glycerol moiety, while the N-acyl chain of N-C6d-DPPE is randomly oriented. These results demonstrate that for N-C16d-DPPE, the N-acyl chain is embedded into the hydrophobic core of the bilayer, while for the short chain derivative the N-acyl chain remains in the lipid headgroup region. The analysis of the carbonyl stretching band and of the amide I band suggests that, for the long N-acyl chain lipid, the ester C=O and the N-H groups are linked by intermolecular hydrogen bonds.

Citations

Nov 7, 2003·Applied and Environmental Microbiology·Hélène GaussierMuriel Subirade
Sep 25, 2012·Biochimica Et Biophysica Acta·Niels WellnerHarald Severin Hansen
May 9, 2014·The Journal of Physical Chemistry. a·Emily S MohnJohn J Breen
Jan 8, 2002·Langmuir : the ACS Journal of Surfaces and Colloids·Xin-Min LiMusti J Swamy

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