n-Butanol production in S. cerevisiae: co-ordinate use of endogenous and exogenous pathways

Applied Microbiology and Biotechnology
R SwidahM P Ashe

Abstract

n-Butanol represents a key commodity chemical and holds significant potential as a biofuel. It can be produced naturally by Clostridia species via the ABE pathway. However, butanol production via such systems can be associated with significant drawbacks. Therefore, substantial efforts have been made toward engineering a suitable industrial host for butanol production. For instance, we previously generated a metabolically engineered Saccharomyces cerevisiae strain that produces ~300 mg/L butanol from combined endogenous and exogenous pathways. In this current study, the endogenous and exogenous pathways of butanol production were further characterised, and their relative contribution to the overall butanol titre was assessed. Deletion of any single component of the exogenous ABE pathway was sufficient to significantly reduce butanol production. Further evidence for a major contribution from the ABE pathway came with the discovery that specific yeast deletion mutants only affected butanol production from this pathway and had a significant impact on butanol levels. In previous studies, the threonine-based ketoacid (TBK) pathway has been proposed to explain endogenous butanol synthesis in ADH1 mutants. However, we find that key mut...Continue Reading

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Citations

Jan 29, 2020·Biotechnology Journal·Olena O KurylenkoAndriy A Sibirny
Mar 11, 2020·World Journal of Microbiology & Biotechnology·Suéllen P H Azambuja, Rosana Goldbeck
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Jun 28, 2020·FEMS Yeast Research·Lisset DuranJosé L Avalos
Mar 28, 2020·Current Opinion in Biotechnology·In Jin ChoSang Yup Lee
Mar 19, 2020·Journal of Agricultural and Food Chemistry·Yanrui MaDongguang Xiao

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Methods Mentioned

BETA
PCR
electrophoresis

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