N-cadherin in neuroblastoma disease: expression and clinical significance.

PloS One
Tim LammensGeneviève Laureys

Abstract

One of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability.

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Jan 31, 2014·In Vitro Cellular & Developmental Biology. Animal·Robert A ReddenEdward J Doolin
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Dec 11, 2021·Biophysical Journal·Themistoklis ZisisStefan Zahler

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Methods Mentioned

BETA
Assay
PCR
protein assay

Software Mentioned

SPSS
Adherex
GraphPad Prism
qbasePLUS
GraphPad

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