N-cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells

Stem Cell Research
Frederik WeinWolfgang Wagner

Abstract

Specific cell-cell junctions between hematopoietic stem cells (HSC) and their niche have been shown to regulate stem cell function. N-cadherin was suggested to play a central role in this process, whereas other studies indicated that it did not play an essential role in the murine model. We have analyzed the role of N-cadherin for interaction between hematopoietic progenitor cells (HPC) and supportive mesenchymal stromal cells (MSC) in a human-human setting. Expression of N-cadherin and of cadherin-11 (osteoblast cadherin) was analyzed in HPC by quantitative RT-PCR, Western blot, and flow cytometry. N-cadherin and cadherin-11 were expressed in HPC at a moderate level, whereas they were not detectable in differentiated cells. Confocal laser scanning microscopy revealed that N-cadherin and beta-catenin are colocalized at the junction of HPC and MSC. siRNA knockdown of N-cadherin or cadherin-11 as well as treatment with the blocking function antibody decreased adhesive interaction of HPC to MSC. Furthermore, knockdown of N-cadherin or blocking function antibody impaired maintenance of long-term culture-initiating cells (LTC-IC) on coculture of HPC and MSC. These results indicate that N-cadherin is involved in the bidirectional int...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J SutherlandC J Eaves
Oct 1, 1995·The Journal of Cell Biology·S ArmeanuG Klein
May 10, 1996·The Journal of Biological Chemistry·K SugimotoT Nakamura
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G LiM Herlyn
Sep 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason A HackneyKateri A Moore
Oct 24, 2003·Nature·L M CalviD T Scadden
Nov 20, 2003·Journal of Cell Science·Beate K StraubWerner W Franke
Jun 17, 2005·Annals of the New York Academy of Sciences·Pierre Charbord, Kateri Moore
Nov 25, 2005·Stem Cells and Development·Matthew A Martin, Mickie Bhatia
Dec 3, 2005·European Journal of Immunology·Stephan GöttigReinhard Henschler
Oct 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·A K PrakasamD E Leckband
Jan 27, 2007·Science·Yukiko M YamashitaMargaret T Fuller
Mar 3, 2007·Annals of the New York Academy of Sciences·Fumio Arai, Toshio Suda
Mar 22, 2007·The Journal of Biological Chemistry·Anna LagergrenMikael Sigvardsson
May 31, 2007·Current Opinion in Hematology·Anthony D Ho, Wolfgang Wagner
Dec 13, 2007·Stem Cell Reviews·Wolfgang Wagner, Anthony D Ho
Dec 28, 2007·Cells, Tissues, Organs·Wolfgang WagnerAnthony D Ho
Feb 15, 2008·The Journal of Biological Chemistry·Jiwang Zhang, Linheng Li
Feb 26, 2008·Cell·Juergen A Knoblich

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Citations

Feb 1, 2013·Advances in Hematology·Felix Nwajei, Marina Konopleva
May 3, 2011·Seminars in Immunopathology·Ian B Copland, Jacques Galipeau
Aug 10, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Anjali MishraRussell S Taichman
Feb 3, 2016·Cell and Tissue Research·Patrick WuchterEric Gottwald
Nov 3, 2015·Archives of Medical Research·Guadalupe R Fajardo-OrduñaJuan J Montesinos
Jul 24, 2012·Cancer Letters·Christoph LutzAnthony D Ho
May 23, 2012·Experimental Hematology·Abraham Zepeda-MorenoAnthony D Ho
Dec 30, 2015·Annals of the New York Academy of Sciences·Melissa van PelKoen Schepers
Sep 19, 2015·European Journal of Haematology·Norbert BlankPatrick Wuchter
Jul 29, 2011·International Journal of Cancer. Journal International Du Cancer·Eike C Buss, Anthony D Ho
Jul 18, 2015·ACS Applied Materials & Interfaces·Nishanth V MenonYuejun Kang
Jul 19, 2014·Trends in Endocrinology and Metabolism : TEM·Pierre J MarieZuzana Saidak
Apr 14, 2016·Current Opinion in Hematology·Madhav Seshadri, Cheng-Kui Qu
Jan 1, 2013·Journal of Clinical Medicine·Matthew M CookKerry Atkinson
Jun 23, 2018·International Journal of Cancer. Journal International Du Cancer·Alexander RühleNils H Nicolay
Feb 13, 2010·Stem Cells and Development·Menno ter HuurneRuurd Torensma
May 3, 2013·The Journal of Clinical Investigation·Claus Kordes, Dieter Häussinger
Dec 4, 2015·PLoS Computational Biology·Kendra S Burbank
Feb 23, 2011·Cell Adhesion & Migration·Abraham Zepeda-MorenoAnthony D Ho
Aug 6, 2020·Frontiers in Cell and Developmental Biology·Rohan Kulkarni, Vaijayanti Kale
Apr 13, 2018·International Journal of Molecular Sciences·Nicola Graham, Bin-Zhi Qian
Aug 6, 2020·Frontiers in Cell and Developmental Biology·Alicia VillatoroLorena Arranz
Mar 27, 2020·The Journal of Cell Biology·Linda ViolGislene Pereira
Apr 5, 2021·Acta Biomaterialia·Chandralekha ChatterjeeCornelia Lee-Thedieck
Mar 9, 2021·Tissue Engineering. Part B, Reviews·Cláudia S OliveiraJoão F Mano
May 22, 2021·Frontiers in Cell and Developmental Biology·Stefania CrippaMaria Ester Bernardo
Apr 24, 2020·ACS Applied Materials & Interfaces·Guillaume Le SauxMark Schvartzman

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