N-cadherin mediated cell contact inhibits germinal vesicle breakdown in mouse oocytes maintained in vitro

Reproduction : the Official Journal of the Society for the Study of Fertility
J J Peluso

Abstract

The effect of granulosa cell contact on the ability of zona-free oocytes to undergo germinal vesicle breakdown (GVBD) was assessed using a granulosa cell co-culture system. Oocytes contacted granulosa cells in a site-specific manner such that their GV was away from the granulosa cells. Also contact with granulosa cells reduced the percentage of oocytes undergoing GVBD from about 40% to 15%. GVBD was inhibited by contact with granulosa cells but not a granulosa cell-secreted product, since oocytes cultured in the same culture, that were adjacent to the granulosa cell monolayer underwent GVBD at the same rate as controls. Similarly, media collected from granulosa cell cultures did not attenuate the rate of GVBD. The ability of granulosa cell contact to inhibit GVBD was equal to that of db-cAMP. Moreover, the ability of granulosa cells to inhibit GVBD was not mimicked by spontaneously immortalized granulosa cells. This cell specificity appeared to be related to N-cadherin, since granulosa cells and oocytes express N-cadherin and a N-cadherin antibody attenuates the effect of granulosa cell contact. The mechanism through which N-cadherin mediated cell contact maintains meiotic arrest is unknown. It is possible that homophilic N-cad...Continue Reading

References

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Citations

Jan 6, 2010·Reproduction, Fertility, and Development·Silvia ModinaAlberto Maria Luciano
Feb 22, 2008·Molecular Reproduction and Development·Michael K SkinnerEric E Nilsson
Jan 22, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Brigitte LefèvreAhmed Ziyyat

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