n-CoDeR concept: unique types of antibodies for diagnostic use and therapy

Expert Review of Molecular Diagnostics
R Carlsson, E Söderlind

Abstract

The n-CoDeR recombinant antibody gene libraries are built on a single master framework, into which diverse in vivo-formed complementarity determining regions (CDRs) are allowed to recombine. These CDRs are sampled from in vivo-processed and proof-read gene sequences, thus ensuring an optimal level of correctly folded and functional molecules. By the modularized assembly process, up to six CDRs can be varied at the same time, providing a possibility for the creation of a hitherto undescribed genetic and functional variation. The n-CoDeR antibody gene libraries can be used to select highly specific, human antibody fragments with specificities to virtually any antigen, including carbohydrates and human self-proteins and with affinities down into the subnanomolar range. Furthermore, combining CDRs sampled from in vivo-processed sequences into a single framework result in molecules exhibiting a lower immunogenicity compared to normal human immunoglobulins, as determined by computer analyses. The distinguished features of the n-CoDeR libraries in the therapeutic and diagnostic areas are discussed.

References

Aug 15, 1991·Nature·T ClacksonG Winter
Mar 24, 1988·Nature·L RiechmannG Winter
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B CochloviusM Little
Aug 1, 2000·Immunology Today·M J Glennie, P W Johnson
Aug 30, 2000·Nature·C M PerouD Botstein

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Citations

Dec 17, 2002·Current Opinion in Biotechnology·Titus Kretzschmar, Thomas von Rüden
May 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dirk PonselKathrin Tissot
Mar 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julia SmithShant Kumar
Aug 19, 2007·Journal of Proteome Research·Johan IngvarssonChrister Wingren

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