N-glycan biosynthesis inhibitors induce in vitro anticancer activity in colorectal cancer cells

Journal of Cellular Biochemistry
Julio Cesar Madureira de-Freitas-JuniorJose A Morgado-Díaz

Abstract

During malignant transformation, changes in the expression profile of glycans may be involved in a variety of events, including the loss of cell-cell and cell-matrix adhesion, migration, invasion, and evasion of apoptosis. Therefore, modulation of glycan expression with drugs has promising therapeutic potential for various cancer types. In this study, we investigated the in vitro anticancer activity of the N-glycan biosynthesis inhibitors (swainsonine and tunicamycin) in cells derived from colorectal cancer (CRC). We also examined whether these inhibitors are able to induce radiosensitization and toxicity when used in combination with cisplatin or irinotecan, two current anticancer drugs. Our results show that treatment with tunicamycin inhibits cellular mechanisms related to the malignant phenotype, such as anchorage-dependent and anchorage-independent colony formation, migration and invasion, in undifferentiated HCT-116 colon cancer cells, whereas swainsonine only inhibits cell migration. We also observed that tunicamycin, but not swainsonine, caused radiosensitivity in HCT-116 cells. Moreover, the combination of swainsonine with cisplatin or irinotecan enhanced their toxicity in HCT-116 cells, while the combination of tunica...Continue Reading

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Citations

Nov 15, 2014·Breast Cancer Research and Treatment·S N OnonyeL Pusztai
Sep 8, 2017·International Journal of Molecular Sciences·Julio Cesar M de-Freitas-JuniorSalomé S Pinho
Nov 6, 2015·Oncotarget·Julio Cesar Madureira de Freitas Junior, José Andrés Morgado-Díaz
Sep 5, 2020·Journal of Medicinal Chemistry·Katsuhiko MitachiMichio Kurosu

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