N-glycan profiling in the study of human aging

Biogerontology
Valerie VanhoorenCui-Ying Chen

Abstract

Most secreted proteins produced by the human body are modified by glycosylation. It is well known that the oligosaccharides (glycans) of glycoproteins are important for initiation of various cellular recognition signals that are essential for the maintenance of the ordered social life of each cell within a multi-cellular organism. The sugar chains can be altered by the physiological or pathophysiological condition of the cell. We describe a detailed protocol for the analysis of N-linked glycans in blood via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE). The key features of this technique are its robustness, high throughput, high sensitivity and reliable quantification. Based on DSA-FACE technology, we previously reported that N-glycan profiling of the human serum shows substantial changes with increasing age in three major N-glycan structures. We proposed that measurement of the N-glycan level changes could provide a surrogate marker for general health or for age-related disease progression, and for monitoring the improvement of health after therapy.

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Citations

Nov 26, 2010·Molecular & Cellular Proteomics : MCP·Maria Lorna A de LeozCarlito B Lebrilla
Apr 30, 2010·Rejuvenation Research·Cuiying Chitty ChenPeter Paul De Deyn
Aug 3, 2011·Age and Ageing·Ning DingYu Li
Feb 15, 2014·Mechanisms of Ageing and Development·Dragana RobajacOlgica Nedić
Feb 23, 2012·Ageing Research Reviews·Fabio Dall'OlioClaudio Franceschi
Aug 31, 2010·Experimental Gerontology·Valerie VanhoorenCuiying Chitty Chen
Sep 29, 2011·Aging Cell·Valerie VanhoorenClaude Libert
Jun 8, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Xue-en LiuCuiying Chitty Chen
Dec 3, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Hong-lian GuiCuiying Chen
Sep 4, 2015·Journal of Proteome Research·Vincenzo BorelliManfred Wuhrer
Jul 21, 2012·Journal of Proteomics·Rita BaroneDomenico Garozzo
Dec 17, 2008·Mechanisms of Ageing and Development·Valerie VanhoorenCuiying Chen
Nov 13, 2015·PloS One·Yuri MiuraUNKNOWN SONIC (Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians)
Oct 17, 2018·AIDS·Davide De FrancescoUNKNOWN the Co-morBidity in Relation to AIDS (COBRA) Collaboration
Feb 6, 2020·Journal of Clinical Medicine·Rocío Fuertes-MartínNúria Amigó
Feb 4, 2016·Oncotarget·Mariangela CateraFabio Dall'Olio
Jan 2, 2021·Mechanisms of Ageing and Development·Chiara PirazziniUNKNOWN PROPAG-AGEING Consortium
Oct 8, 2019·Mechanisms of Ageing and Development·Dragana RobajacOlgica Nedić
Jun 3, 2021·International Journal of Molecular Sciences·Beatrix PatonNúria Canela
Oct 23, 2010·Analytical Chemistry·Rajiv BharadwajAnup K Singh

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