N-glycans and metastasis in galectin-3 transgenic mice

Biochemical and Biophysical Research Communications
Shyam K MoreRajiv D Kalraiya

Abstract

Poly-N-acetyl-lactosamine (polyLacNAc) on N-glycans facilitate lung specific metastasis of melanoma cells by serving as high affinity ligands for galectin-3, expressed in highest amounts in the lungs, on almost all its tissue compartments including on the surface of vascular endothelium. PolyLacNAc not only aids in initial arrest on the organ endothelium but in all the events of extravasation. Inhibition of polyLacNAc synthesis, or competitive inhibition of its interaction with galectin-3 all inhibited these processes and experimental metastasis. Transgenic galectin-3 mice, viz., gal-3(+/+) (wild type), gal-3(+/-) (hemizygous) and gal-3(-/-) (null) have been used to prove that galectin-3/polyLacNAc interactions are indeed critical for lung specific metastasis. Gal-3(+/-) mice which showed <50% expression of galectin-3 on the lungs also showed proportionate decrease in the number of B16F10 melanoma metastatic colonies affirming that galectin-3 and polyLacNAc interactions are indeed key determinants of lung metastasis. However, surprisingly, the number and size of metastatic colonies in gal-3(-/-) mice was very similar as that seen in gal-3(+/+) mice. The levels of lactose binding lectins on the lungs and the transcripts of other...Continue Reading

References

Feb 1, 1996·The Journal of Experimental Medicine·L BianconeI Stamenkovic
Dec 2, 1999·Biochimica Et Biophysica Acta·J W DennisC E Warren
Jun 20, 2002·Trends in Immunology·Gabriel A RabinovichStefano Iacobelli
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Sep 12, 2002·Biochimica Et Biophysica Acta·Jun HirabayashiKen-ichi Kasai
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brianna N StillmanLinda G Baum
Feb 16, 2006·Biochimica Et Biophysica Acta·Jerka DumicMirna Flögel
Aug 8, 2006·Nature Medicine·Patricia S Steeg
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Sep 25, 2007·Clinical & Experimental Metastasis·Adit Ben-Baruch
Dec 10, 2008·Autoimmunity Reviews·Amy DhirapongFu-Tong Liu
Apr 15, 2009·Nature Reviews. Immunology·Gabriel A Rabinovich, Marta A Toscano
Mar 29, 2011·Clinical & Experimental Metastasis·Gordana RadosavljevicMiodrag L Lukic
Jul 2, 2014·Frontiers in Oncology·Anneliese Fortuna-CostaMauro S G Pavão

❮ Previous
Next ❯

Citations

Oct 8, 2015·Molecular Immunology·Aparna D ChaudhariShubhada V Chiplunkar
Nov 2, 2016·Journal of the American Society of Nephrology : JASN·Mingzhu NieMatthias T F Wolf
Aug 17, 2016·BMC Cancer·Jonathas Xavier PereiraMárcia Cury El-Cheikh
Mar 21, 2017·Seminars in Cancer Biology·Leticia Oliveira-FerrerKarin Milde-Langosch
May 1, 2016·Molecular and Cellular Biochemistry·Shyam K MoreRajiv D Kalraiya
Aug 28, 2021·Cells·Chiara De VellisBarbara Stecca

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis