N-Glycoproteomics of Human Seminal Plasma Glycoproteins

Journal of Proteome Research
Mayank SaraswatRisto Renkonen

Abstract

Seminal plasma aids sperm by inhibiting premature capacitation, helping in the intracervical transport and formation of an oviductal sperm reservoir, all of which appear to be important in the fertilization process. Epitopes such as Lewis x and y are known to be present on seminal plasma glycoproteins, which can modulate the maternal immune response. It is suggested by multiple studies that seminal plasma glycoproteins play, largely undiscovered, important roles in the process of fertilization. We have devised a strategy to analyze glycopeptides from a complex, unknown mixture of protease-digested proteins. This analysis provides identification of the glycoproteins, glycosylation sites, glycan compositions, and proposed structures from the original sample. This strategy has been applied to human seminal plasma total glycoproteins. We have elucidated glycan compositions and proposed structures for 243 glycopeptides belonging to 73 N-glycosylation sites on 50 glycoproteins. The majority of the proposed glycan structures were complex type (83%) followed by high-mannose (10%) and then hybrid (7%). Most of the glycoproteins were either sialylated, fucosylated, or both. Many Lewis x/a and y/b epitopes bearing glycans were found, sugg...Continue Reading

References

Nov 10, 1995·The Journal of Biological Chemistry·K el OuagariH Benoist
Dec 13, 1996·The Journal of Biological Chemistry·H R MorrisG F Clark
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ben J AppelmelkYvette van Kooyk
Dec 1, 2006·Nucleic Acids Research·Fiona M McCarthyShane C Burgess
Feb 15, 2008·Glycobiology·Sakari JoenvääräRisto Renkonen
Sep 18, 2008·The Journal of General Virology·Marein A W P de JongTeunis B H Geijtenbeek
Mar 10, 2009·Glycobiology·Hannu PeltoniemiRisto Renkonen
May 22, 2010·Journal of Separation Science·Mashkoor AlamSavita Yadav
Oct 21, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan SabatteSebastian Amigorena
Aug 15, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·J P RickardS P de Graaf
Sep 25, 2014·Asian Journal of Andrology·Beata OlejnikMirosława Ferens-Sieczkowska
Dec 3, 2014·Molecular & Cellular Proteomics : MCP·Mayank SaraswatRisto Renkonen
Mar 13, 2015·Biology of Reproduction·Viswanadhapalli SuryavathiJohn C Herr

❮ Previous
Next ❯

Citations

Jan 2, 2019·Molecular & Cellular Proteomics : MCP·Ryan DosselliBoris Baer
Nov 16, 2019·Expert Review of Proteomics·Valter Luiz MacielRicardo Pimenta Bertolla
Oct 20, 2017·Basic and Clinical Andrology·Jana VitkuRichard Hampl
Mar 30, 2020·Molecular Reproduction and Development·Ewa Janiszewska, Ewa Maria Kratz
Nov 18, 2020·International Journal of Molecular Sciences·Fengli WangShuiqiao Yuan
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Ryan DosselliBoris Baer
Sep 3, 2020·Journal of Proteome Research·Rongxia LanShisheng Sun
Aug 4, 2021·Scientific Reports·Ewa JaniszewskaEwa Maria Kratz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.