N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding

International Journal of Cancer. Journal International Du Cancer
Manja WobusGabriela Aust

Abstract

CD97 is an EGF-TM7 receptor found on various carcinomas where expression levels correlate with dedifferentiation and tumor stage, smooth muscle cells and leukocytes. CD97 acts as an adhesion molecule by binding to its cellular ligand, CD55. In this study, we demonstrate that 2 immunodominant CD97 epitopes are not equally present in the various cell types. Differences were apparent in gastrointestinal tumors and smooth muscle cells where monoclonal antibodies (mAbs) to the first epidermal growth factor (EGF) domain (CD97(EGF)) showed a more restricted staining pattern than mAbs to the stalk region (CD97(stalk)). This discrepancy was not detectable in cultured gastrointestinal tumor cell lines. In fact, the selection of the CD97 mAb influences the result of clinical studies. Thus, we clarified the reason(s) for these differences in CD97 mAb staining on various cell types. We provide evidence that epitope accessibility for CD97(EGF) mAbs depends on N-glycosylation. Immunoprecipitation of CD97 from the Colo 205 tumor cell line revealed the established 78 and 83 kDa products, while a 52 and 57 kDa band were obtained from smooth muscle cells. N-glycosidase F reduced the size of CD97 in Colo 205 cells to 52-57 kDa. Culturing these cel...Continue Reading

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Citations

Mar 27, 2010·International Journal of Colorectal Disease·Shao-Liang HanQi-Qiang Zeng
Jan 13, 2006·Cell and Tissue Research·Gabriela AustManja Wobus
May 23, 2013·Science Signaling·Tobias LangenhanJörg Hamann
May 2, 2012·Journal of Biomedicine & Biotechnology·Jinsheng WuJianhuai Zhang
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Jun 14, 2015·The International Journal of Biochemistry & Cell Biology·Cheng-Chih HsiaoGabriela Aust
May 2, 2016·Biochemical Pharmacology·Saskia NijmeijerRob Leurs
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Aug 30, 2019·Nature Reviews. Drug Discovery·Frederic BassilanaMarie-Gabrielle Ludwig
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Jan 3, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elke WandelGabriela Aust
Aug 24, 2013·International Journal of Oncology·Michael SafaeeAndrew T Parsa
Nov 5, 2019·The Journal of Clinical Investigation·Xiaoyan XuHan Liang

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