N-glycosylation of colorectal cancer tissues: a liquid chromatography and mass spectrometry-based investigation.

Molecular & Cellular Proteomics : MCP
Crina I A BalogManfred Wuhrer

Abstract

Colorectal cancer is the third most common cancer worldwide with an annual incidence of ~1 million cases and an annual mortality rate of ~655,000 individuals. There is an urgent need for identifying novel targets to develop more sensitive, reliable, and specific tests for early stage detection of colon cancer. Post-translational modifications are known to play an important role in cancer progression and immune surveillance of tumors. In the present study, we compared the N-glycan profiles from 13 colorectal cancer tumor tissues and corresponding control colon tissues. The N-glycans were enzymatically released, purified, and labeled with 2-aminobenzoic acid. Aliquots were profiled by hydrophilic interaction liquid chromatography (HILIC-HPLC) with fluorescence detection and by negative mode MALDI-TOF-MS. Using partial least squares discriminant analysis to investigate the N-glycosylation changes in colorectal cancer, an excellent separation and prediction ability were observed for both HILIC-HPLC and MALDI-TOF-MS data. For structure elucidation, information from positive mode ESI-ion trap-MS/MS and negative mode MALDI-TOF/TOF-MS was combined. Among the features with a high separation power, structures containing a bisecting GlcNA...Continue Reading

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Methods Mentioned

BETA
glycosylation
PCA
xenograft
sulfotransferase

Software Mentioned

MATLAB
GraphPad Prism
PLS
SIMCA P +
ClinPro Tools
FlexAnalysis
FlexControl
DA

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