PMID: 9634799Jun 1, 1995Paper

N-glycosylation of recombinant human interferon-gamma produced in different animal expression systems

Bio/technology
D JamesN Jenkins

Abstract

Recombinant human interferon-gamma (IFN-gamma) was expressed in Chinese hamster ovary cells, baculovirus-infected Sf9 insect cells and the mammary gland of transgenic mice. The N-linked carbohydrate populations associated with both Asn25 and Asn97 glycosylation sites were characterized by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) in combination with exoglycosidase array sequencing. A site-specific analysis of dual (2N) and single (1N) site-occupancy variants of IFN-gamma derived from Chinese hamster ovary cells showed that N-glycans were predominantly of the complex bi- and triantennary type. Although Asn25-linked glycans were substituted with a core fucose residue, Asn97 N-glycans were predominantly non-fucosylated, and truncated complex and high-mannose oligosaccharide chains were also evident. Transgenic mouse derived IFN-gamma exhibited considerable site-specific variation in N-glycan structures. Asn25-linked carbohydrates were of the complex, core fucosylated type, Asn97-linked carbohydrates were mainly of the oligomannose type, with smaller proportions of hybrid and complex N-glycans. Carbohydrates associated with both glycosylation sites of IFN-gamma from Sf9 insect cells were mainly tri-ma...Continue Reading

References

Feb 1, 1991·Applied Microbiology and Biotechnology·P M HayterA T Bull
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J EdgeR A Dwek
Dec 20, 1991·Cell·K Drickamer
Dec 1, 1991·Archives of Biochemistry and Biophysics·M TandaiA Kobata
May 2, 1986·European Journal of Biochemistry·J H MutsaersJ F Vliegenthart
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Jun 1, 1984·Biochemical Society Transactions·E Bause
Nov 1, 1994·Protein Science : a Publication of the Protein Society·J M Buchanan
Jul 1, 1993·Biotechnology Progress·M J Gramer, C F Goochee
May 1, 1994·Enzyme and Microbial Technology·N Jenkins, E M Curling
Oct 15, 1993·Analytical Chemistry·M C HubertyS A Martin
Mar 15, 1993·FEBS Letters·V N DobrovolskyO A Larionov

❮ Previous
Next ❯

Citations

Mar 21, 2001·Biotechnology and Bioengineering·K N BakerD C James
Sep 18, 1998·Electrophoresis·N H Packer, M J Harrison
Feb 1, 1996·Protein Science : a Publication of the Protein Society·D C JamesR B Freedman
Apr 14, 2010·The Protein Journal·Christopher J VavrickaJianyong Li
Jul 11, 2007·In Vitro Cellular & Developmental Biology. Animal·Su-Jeong HwangGyun Min Lee
Oct 1, 1995·Current Opinion in Biotechnology·K L WilliamsM B Slade
Dec 1, 1995·Current Opinion in Biotechnology·K Koths
Apr 1, 1996·Current Opinion in Biotechnology·N Jenkins
Oct 1, 1996·Current Opinion in Biotechnology·Y Echelard
Aug 1, 1996·Nature Biotechnology·N JenkinsD C James
Mar 9, 1999·Preparative Biochemistry & Biotechnology·M W WolffR J Linhardt
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·O V LagutinO A Larionov
Jun 10, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A Hooker, D James
Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Fabienne RauwThierry van den Berg
Dec 4, 2003·Bioscience, Biotechnology, and Biochemistry·Tatsuya KatoEnoch Y Park
Jun 25, 1998·Electrophoresis·N H PackerA A Gooley
Jan 1, 2014·The FEBS Journal·Yasuhiro ShibasakiTeruyuki Nakanishi
Jun 18, 2010·The Journal of General Virology·Chumporn SoowannayanPeter J Walker
Dec 14, 2004·Biotechnology and Bioengineering·Danny Chee Furng WongMiranda Gek Sim Yap
Nov 16, 2007·Journal of Cellular Biochemistry·Pamela A NortonTimothy M Block
Jan 29, 2000·FEBS Letters·Y NishikawaT Mikami

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.