PMID: 3745141Jun 1, 1986Paper

N-hydroxyarylamine O-acetyltransferase in hamster liver: identity with arylhydroxamic acid N,O-acetyltransferase and arylamine N-acetyltransferase

Journal of Biochemistry
K SaitoR Kato

Abstract

N-Hydroxyarylamine O-acetyltransferase, arylhydroxamic acid N,O-acetyltransferase, and arylamine N-acetyltransferase in hamster liver cytosol were co-purified almost to electrophoretical homogeneity by ion exchange chromatography on DEAE-cellulose, gel filtration on Cellulofine GCL-2000-sf and high-performance KB-hydroxyapatite chromatography. The molecular weight of the acetyltransferase was estimated to be 33,000 by gel filtration and SDS-polyacrylamide gel electrophoresis. The three acetyltransferase activities were inhibited by iodoacetamide, pentachlorophenol, and 1-nitro-2-naphthol. Furthermore, 2-aminofluorene, a substrate for arylamine N-acetyltransferase, inhibited the reactions of N-hydroxyarylamine O-acetyl transfer and arylhydroxamic acid N,O-acetyl transfer. These results suggest that the same enzyme catalyzes the three types of acetyl transfer reactions. The acetyltransferase could activate N-hydroxyarylamines, such as 2-hydroxyamino-6-methyldipyrido[1,2-alpha:3',2'-d]imidazole, 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole, and N-hydroxy-2-aminofluorene, to the corresponding N-acetoxyarylamines, which are capable of binding to nucleic acid. Polyguanylic acid was most efficiently modified by the N-acetoxyarylamin...Continue Reading

Citations

Dec 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D W Hein
Jul 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P E HannaM E McCormack
Feb 15, 1989·European Journal of Biochemistry·E C Webb
Mar 11, 2000·Drug Metabolism Reviews·R Kato, Y Yamazoe
Jun 30, 2000·Toxicology and Applied Pharmacology·J J Yourick, R L Bronaugh
Oct 12, 1995·Biochemical Pharmacology·H NakuraT Kamataki
Sep 15, 1990·Biochemical Pharmacology·M J WickP E Hanna
Aug 3, 1988·Biochimica Et Biophysica Acta·D W Hein

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