N-linked glycoprotein synthesis and transport during G1 are necessary for astrocytic proliferation

Journal of Neuroscience Research
S Ishii, J J Volpe

Abstract

The proliferation of astrocytes, purified by a selective detachment technique from mixed glial primary cultures derived from newborn rat cerebrum, was studied. The cells were synchronized by first inducing a quiescent state by removing fetal calf serum (FCS) from the culture medium for 2 days; reversal of the quiescent state by return of serum to the culture medium caused a marked increase in DNA synthesis 12-24 hr later. 2-Deoxyglucose, an inhibitor of dolichol-linked oligosaccharide and thereby N-linked glycoprotein biosynthesis, prevented not only an increase in glycoprotein biosynthesis in G1 phase of the cell cycle but also the burst of DNA synthesis that followed during S phase. Addition of mannose to the culture medium prevented the inhibitions by deoxyglucose of both glycoprotein and DNA syntheses. These data indicated an obligatory relationship in astrocytes between dolichol-linked glycoprotein synthesis and DNA synthesis. To determine whether transport of the newly synthesized glycoproteins to the plasma membrane for incorporation therein or for secretion were necessary for DNA synthesis and astrocytic proliferation, we studied cells treated with monensin, an ionophore for monovalent cations, and an inhibitor of intra...Continue Reading

References

Jan 1, 1978·Annual Review of Neuroscience·P H Patterson
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N UchidaM L Tanzer
Jan 1, 1979·Methods in Enzymology·T Ashihara, R Baserga
Nov 1, 1977·The Journal of Experimental Medicine·A M Tartakoff, P Vassalli
Jul 1, 1976·Journal of Cellular Physiology·J C BartholomewP Ross
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R P Saneto, J de Vellis
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F AloisiG Levi
Jan 1, 1987·Methods in Enzymology·A D Elbein
Jan 1, 1986·Journal of Neuroscience Research·S Bhat, S E Pfeiffer
Jan 1, 1984·Annual Review of Neuroscience·D K Berg
Feb 19, 1981·The New England Journal of Medicine·R Baserga
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R E ScottK Yun
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Dec 1, 1980·The Journal of Cell Biology·L KääriäinenK Penttinen
Jan 1, 1983·Experimental Brain Research·L BologaN Herschkowitz
Sep 1, 1984·Brain Research·P A Eccleston, D H Silberberg
Jan 1, 1983·Methods in Enzymology·W Lennarz
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y A BardeH Thoenen
Mar 1, 1965·Archives of Biochemistry and Biophysics·K D MUNKRES, F M RICHARDS

❮ Previous
Next ❯

Citations

Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Kukuruzinska, K Lennon
Dec 24, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C L BaconC M Regan
Feb 14, 2021·Reproduction in Domestic Animals = Zuchthygiene·Monika JamiołMarta Kankofer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.