N-linked glycosylation selectively regulates autonomous precursor BCR function.

Nature Immunology
Rudolf UbelhartHassan Jumaa

Abstract

Developing B cells express distinct classes of B cell antigen receptors (BCRs) that differ in their heavy chain (HC). Although only muHC is expressed in early stages, deltaHC-containing BCRs dominate on the surface of mature B cells. The reason for the tightly regulated expression of these receptors is poorly understood. Here we show that muHC was specifically required for precursor BCR (pre-BCR) function and that deltaHC was unable to form a functional pre-BCR. A conserved asparagine (N)-linked glycosylation site at position 46 (N46) in the first conserved domain of muHC was absolutely required for pre-BCR function, and swapping that domain with deltaHC resulted in a functional deltaHC-containing pre-BCR. When tested in the context of the BCR, muHC with a mutant N46 showed normal function, which indicated that N46-glycosylation is specifically required for pre-BCR function. Our results suggest an unexpected mode of pre-BCR function, in which binding of the surrogate light chain to N46 mediates autonomous crosslinking and, concomitantly, receptor formation.

References

May 29, 1992·Cell·D KitamuraK Rajewsky
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R TischN Hozumi
Jan 1, 1997·Annual Review of Immunology·T F TedderJ H Kehrl
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y MinegishiM E Conley
Sep 11, 2002·Seminars in Immunology·Peter D BurrowsMax D Cooper
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laurent GauthierClaudine Schiff
Nov 19, 2002·Nature Immunology·Alexandra FlemmingHassan Jumaa
Dec 3, 2002·The Journal of Biological Chemistry·Anne Marie MoodyEllis L Reinherz
Aug 16, 2003·Science·Hedda WardemannMichel C Nussenzweig
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Harald BradlHans-Martin Jäck
Aug 23, 2003·Immunity·Yu-wen Su, Hassan Jumaa
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wolfgang SchuhHans-Martin Jäck
Nov 26, 2003·The Journal of Experimental Medicine·Yu-Wen SuHassan Jumaa
Jan 3, 2006·Seminars in Immunology·Jamie K Geier, Mark S Schlissel
Aug 5, 2006·Science·Yoshikatsu KanekoJeffrey V Ravetch
Dec 14, 2006·European Journal of Immunology·Bettina StorchHassan Jumaa
Apr 14, 2007·Science·Alexander J BankovichK Christopher Garcia
Mar 29, 2008·Annual Review of Immunology·Falk Nimmerjahn, Jeffrey V Ravetch

❮ Previous
Next ❯

Citations

Nov 16, 2011·The Journal of Biological Chemistry·Wenzhe LiNaoyuki Taniguchi
Feb 1, 2012·The Journal of Experimental Medicine·Alabbas AlkhatibHassan Jumaa
Feb 18, 2012·Blood·Freda K Stevenson, George T Stevenson
Feb 21, 2014·Zeitschrift für Rheumatologie·M P BachH Jumaa
Oct 24, 2015·Cellular and Molecular Life Sciences : CMLS·Maria De GrandisMichel Aurrand-Lions
Apr 16, 2013·Protein Expression and Purification·Marcie B Jaffee, Barbara Imperiali
Apr 9, 2013·Hematology/oncology Clinics of North America·Yair HerishanuAdrian Wiestner
Sep 10, 2015·European Journal of Immunology·Rudolf Übelhart, Hassan Jumaa
Mar 9, 2012·Current Opinion in Immunology·Sebastian Herzog, Hassan Jumaa
Feb 14, 2012·Current Opinion in Biotechnology·Wolfgang W A Schamel, Michael Reth
Mar 1, 2011·Current Opinion in Immunology·Kang Chen, Andrea Cerutti
Aug 23, 2012·Scandinavian Journal of Immunology·N Almqvist, I-L Mårtensson
Nov 18, 2014·Immunity, Inflammation and Disease·Kolandaswamy AnbazhaganKaïss Lassoued
May 24, 2011·European Journal of Immunology·Cathrin EschbachHassan Jumaa
Apr 3, 2016·Journal of the American Society for Mass Spectrometry·Edward S X MohNicolle H Packer
Jun 19, 2013·International Reviews of Immunology·Laurens P Kil, Rudi W Hendriks
Jun 4, 2011·Expert Opinion on Therapeutic Targets·Rudi W HendriksFrank Jt Staal
Mar 10, 2018·Expert Review of Proteomics·Arun V Everest-DassNicolle H Packer
Mar 25, 2014·Nature Reviews. Cancer·Rudi W HendriksLaurens P Kil
May 8, 2018·European Journal of Immunology·Cindy GutzeitAndrea Cerutti
Feb 20, 2018·Molecular Cancer·Simar Pal SinghRudi W Hendriks
Jan 6, 2018·Nature Reviews. Cancer·Markus Müschen
Jul 16, 2019·Journal of Leukocyte Biology·Rebecca Blandino, Nicole Baumgarth
Apr 22, 2011·Blood·Daniel CorcosLouise S Matheson
Feb 5, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ella Levit-ZerdounElias Hobeika
Nov 30, 2018·Frontiers in Immunology·Yi-Ling SuHui Geng
Oct 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Van B T TaRudi W Hendriks
May 23, 2014·Oncotarget·Brice LaffleurMichel Cogné
Jul 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wenzhao MengEline T Luning Prak
Aug 15, 2018·International Journal of Molecular Sciences·Michel Aurrand-Lions, Stéphane J C Mancini
Mar 7, 2019·Science Signaling·Isabel WilhelmWinfried Römer
Dec 1, 2018·Frontiers in Immunology·Thomas H Winkler, Inga-Lill Mårtensson
Sep 17, 2021·Journal of Leukocyte Biology·Anwen RenChaohong Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.