Abstract
Increased levels of interleukin (IL)-1β and its gene expression are implicated in the etiology of Alzheimer's disease (AD). IL-1β activates microglia and stimulates glutamatergic N-methyl-d-aspartate receptor NMDA receptor expression, thereby disturbing intracellular Ca2+ homeostasis. Ca2+ disequilibrium, in turn, may trigger endoplasmic reticulum (ER) stress, contributing to overall excitotoxicity and neuronal death that evoke AD. However, it is unclear whether IL-1β-induced neuronal apoptosis is mediated by the glutamatergic system, ER stress and/or Ca2+ dysfunction. The present study investigated the role of NMDA receptor (NMDAR) in ER stress and IL-1β-evoked neuronal death by assessing NMDAR-induced Ca2+ overload and NMDA-mediated ER stress. Male Long Evans rats were treated with IL-1β (with or without NMDAR antagonist MK801) injected intracerebroventricularly for 8days. Glutamate concentration was measured by HPLC, and mRNA and protein expression of microglial biomarkers and NMDAR, as well as markers of Ca2+ overload (caplain2) and ER stress (glucose-regulated protein 78, GRP78, and C/EBP homologous protein-10, CHOP), were assessed by real-time PCR and western blot. Apoptosis was also evaluated in the hippocampal neurons u...Continue Reading
Citations
Dec 5, 2018·Free Radical Research·Wei LiuYu Deng
Jul 19, 2019·Journal of Cellular Physiology·Gai-Li JiaHong Cao
Sep 8, 2017·Frontiers in Immunology·Isabella G OlmoFabiola M Ribeiro
Jul 17, 2018·Neurotoxicity Research·Ankita BhardwajTanzeer Kaur
Dec 26, 2018·Frontiers in Immunology·Cuiqin FanShu Yan Yu
Jul 11, 2019·Neural Regeneration Research·Nan-Xing YiTing Zhang
Jun 27, 2020·Psychopharmacology·Chao LuoXiangping Li
Oct 17, 2017·The Journal of Physiology·Meng Zhang, Javier E Stern
Nov 1, 2017·Frontiers in Neurology·Jian QiuBo Bai
May 4, 2018·Journal of Neuroinflammation·Bian-Rong WangYing-Dong Zhang
Jan 23, 2019·Frontiers in Cellular Neuroscience·Cuiqin FanShu Yan Yu
Jun 11, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·E Sherwood BrownKendra Malone
Dec 5, 2019·Scientific Reports·Kai-Xin YaoPing Wang
Dec 12, 2018·Andrologia·Patrick Brice Defo DeehUmar Zayyanu Usman
Apr 19, 2019·Journal of Neuroinflammation·Diego Rolando Hernández-EspinosaJulio Morán
Nov 8, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Amany TawfikMohamed Al-Shabrawey
Oct 30, 2020·Neuropsychiatric Disease and Treatment·Kun ZhangWeidong Shen
Mar 27, 2021·Frontiers in Pharmacology·Noelia RuzafaElena Vecino
Mar 13, 2018·Journal of Neuroimmunology·Virginia D McLaneLing Cao
Aug 8, 2021·Molecular Neurobiology·Yongping ChenHonggang Fan