PMID: 8592141Feb 1, 1996Paper

N-methyl-D-aspartate receptor proteins NR2A and NR2B are differentially distributed in the developing rat central nervous system as revealed by subunit-specific antibodies

Journal of Neurochemistry
C Portera-CailliauL J Martin

Abstract

We have identified the regional distributions and developmental expression of NMDA-receptor proteins NR2A and NR2B in rat CNS, using two subunit-specific affinity-purified polyclonal antibodies that recognize NR2A and NR2B. In western blots of cells transfected with NR2A or NR2B cDNAs, and of brain homogenates, each antibody detects a single predominant 172-kDa protein corresponding to its homologous subunit. Both subunits are glycoproteins that are enriched in synaptic membranes. In adult rat CNS, NR2A and NR2B are enriched in cortex and hippocampus but are present in other forebrain regions. In hindbrain, NR2A is present at low levels but NR2B is barely detectable. These subunits are differentially expressed in postnatal CNS development. In cortex and striatum, NR2A is absent at birth but expression increases thereafter, whereas NR2B is expressed at nearly adult levels during forebrain development. In hindbrain, low levels of NR2A are present throughout development, whereas NR2B is expressed only transiently in the first postnatal weeks. These results suggest that native NMDA receptors are modulated by NR2A and NR2B in adult forebrain but not appreciably in hindbrain. In contrast, during early postnatal development, NR2B may ...Continue Reading

Citations

Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R Sircar
Jul 19, 2002·Synapse·Nicole L SchrammDanny G Winder
Jan 16, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michelle E SchoberRobert H Lane
Apr 12, 2013·Journal of Neurophysiology·Carrie R FerrarioPaulette B Goforth
Aug 3, 2002·Journal of Neurochemistry·Barbara AhlemeyerJosef Krieglstein
Aug 1, 1997·The European Journal of Neuroscience·K UllensvangN C Danbolt
Sep 6, 2003·The European Journal of Neuroscience·Amanda J LawPaul J Harrison
Oct 27, 2004·Journal of Inherited Metabolic Disease·S Varadkar, R Surtees
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·T BlankJ Spiess
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·L J MartinR J Traystman
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