N-methyl D-aspartate receptor subtype 2B antagonist, Ro 25-6981, attenuates neuropathic pain by inhibiting postsynaptic density 95 expression

Scientific Reports
Ling-Er HuangYong-Xing Yao

Abstract

Postsynaptic density-95 (PSD-95) is a synaptic scaffolding protein that plays a crucial role in the development of neuropathic pain. However, the underlying mechanism remains unclear. To address the role of PSD-95 in N-methyl-D-aspartate receptor subtype 2B (NR2B) -mediated chronic pain, we investigated the relationship between PSD-95 activation and NR2B function in the spinal cord, by using a rat model of sciatic nerve chronic constriction injury (CCI). We demonstrate that the expression levels of total PSD-95 and cAMP response element binding protein (CREB), as well as phosphorylated NR2B, PSD-95, and CREB, in the spinal dorsal horn, and the interaction of NR2B with PSD-95 were increased in the CCI animals. Intrathecal injection of the selective NR2B antagonist Ro 25-6981 increased paw withdrawal latency, in a thermal pain assessment test. Moreover, repeated treatment with Ro 25-6981 markedly attenuated the thermal hypersensitivity, and inhibited the CCI-induced upregulation of PSD-95 in the spinal dorsal horn. Furthermore, intrathecal injection of the PSD-95 inhibitor strikingly reversed the thermal and mechanical hyperalgesia. Our results suggest that blocking of NR2B signaling in the spinal cord could be used as a therapeu...Continue Reading

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Citations

Jun 20, 2020·Journal of Molecular Neuroscience : MN·Yuetao MaMingcang Wang
Oct 26, 2020·Molecular Neurobiology·Yanling LiangYinghong Tian
Mar 7, 2021·International Journal of Molecular Sciences·Yu-Jung ChengHsien-Yuan Lane
Mar 13, 2021·Progress in Neurobiology·Rabia Bouali-BenazzouzPascal Fossat

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Methods Mentioned

BETA
immunoprecipitation
co-IP
electrophoresis

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